Production and characterization of agonistic monoclonal antibodies against chicken CD40

被引：6
作者：
Chen, Chang-Hsin [1 ]
Abi-Ghanem, Daad [1 ]
Njongmeta, Leo [3 ]
Bray, Jocelyn [3 ]
Mwangi, Waithaka [3 ]
Waghela, Suryakant D. [3 ]
McReynolds, Jackson L. [4 ]
Ing, Nancy H. [2 ]
Berghman, Luc R. [1 ]
机构：
[1] Texas A&M Univ, Dept Poultry Sci, College Stn, TX 77843 USA
[2] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA
[3] Texas A&M Univ, Dept Vet Pathobiol, College Stn, TX 77843 USA
[4] ARS, USDA, Agr Res Ctr, College Stn, TX 77845 USA
来源：
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY | 2010年 / 34卷 / 11期
关键词：
Chicken; CD40; CD154; Monoclonal antibody; B-cells; Macrophages; HUMAN B-CELLS; NITRIC-OXIDE PRODUCTION; NECROSIS-FACTOR-ALPHA; IN-VIVO; IMMUNE-RESPONSES; BABESIA-BOVIS; LIGAND; ACTIVATION; MACROPHAGES; ANTIGEN;
D O I：
10.1016/j.dci.2010.06.014
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
CD40 is mainly expressed by professional antigen-presenting cells (APCs). Its ligand,CD40L, is transiently expressed on activated CD4(+) T-cells. CD40-CD40L interactions mediate T-cell help to APCs and provide crucial signals for affinity maturation and B-cell class switching. In mammals, agonistic monoclonal anti-CD40 antibodies (mAbs) mimic the effects of CD40L on APCs, leading to enhanced T-cell priming and expansion, increased antibody production and class switching. In this study, we describe agonistic anti-chicken CD40 mAb 2C5. This mAb detected CD40 on primary chicken B-cells and macrophages. DT40 B-cells, and HD11 macrophages, induced NO synthesis in HD11 macrophages, and stimulated DT40 B-cell proliferation. These observations demonstrated at least partial functional equivalence of 2C5 to chicken CD154. This mAb may therefore constitute a new tool to study the role of CD40 in the chicken immune system, and its agonistic effects suggest that it could also be used as an adjuvant. (C) 2010 Elsevier Ltd. All rights reserved.
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页码：1141 / +
页数：5
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