Application of protein-fragment complementation assays in cell biology

被引：67
|
作者：
Remy, Ingrid [1 ]
Michnick, Stephen W. [1 ]
机构：
[1] Univ Montreal, Dept Biochim, Montreal, PQ H3C 3J7, Canada
来源：
BIOTECHNIQUES | 2007年 / 42卷 / 02期
关键词：
D O I：
10.2144/000112396
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We have developed a general experimental strategy that enables the quantitative detection of dynamic protein-protein interactions in intact living cells, based on protein-fragment complementation assays (PCAs). In this method, protein-protein interactions are coupled to refolding of enzymes from cognate fragments where reconstitution of enzyme activity acts as the detector of a protein interaction. Here we discuss the application of PCA to different aspects of cell biology.
引用
收藏
页码：137 / +
页数：5
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