Development of a near-infrared fluorescent sensor with a large Stokes shift for sensing pyrophosphate in living cells and animals

被引：18
作者：
Yang, Shengjun [1 ]
Feng, Weiyong [1 ]
Feng, Guoqiang [1 ]
机构：
[1] Cent China Normal Univ, Key Lab Pesticide & Chem Biol, Hubei Int Sci & Technol Cooperat Base Pesticide &, Minist Educ,Coll Chem, Wuhan 430079, Hubei, Peoples R China
来源：
ANALYTICA CHIMICA ACTA | 2018年 / 1034卷
基金：
中国国家自然科学基金;
关键词：
Pyrophosphate; Near-infraredfluorescent sensor; Large Stokes shift; Bioimaging; In vitro and in vivo detection; INDICATOR DISPLACEMENT ASSAYS; HYDROGEN-SULFIDE; IN-VIVO; TURN-ON; INORGANIC PYROPHOSPHATE; ANION RECOGNITION; AQUEOUS-SOLUTION; ZINC COMPLEX; FAR-RED; PROBE;
D O I：
10.1016/j.aca.2018.05.076
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The detection of pyrophosphate (PPi) in living systems has attracted considerable attention in recent years due to its biological significance. While many fluorescent PPi sensors were developed with emission changes in the visible region, near-infrared (NIR) fluorescent sensors for PPi are rather scarce. In this paper, a dicyanomethylene-benzopyran (DCMB) based phenol-bridged dinuclear Zn(II)-DPA (DPA: dipicolylamine) complex was prepared, which was found to be a promising NIR fluorescent sensor for PPi. This sensor features a remarkable large Stokes shift (>150 nm) and shows colorimetric and NIR fluorescence changes for PPi with high selectivity and sensitivity in 100% aqueous solution. The detection limit of this sensor for PPi was determined to be as low as 42 nM. In addition, this sensor exhibits low cytotoxicity and can be conveniently employed for bioimaging PPi in living cells and animals, indicating it has great potential for in vitro and in vivo detection of PPi. (C) 2018 Elsevier B.V. All rights reserved.
引用
收藏
页码：119 / 127
页数：9
相关论文
共 63 条
[1]   The phenanthroimidazole-based dizinc(II) complex as a fluorescent probe for the pyrophosphate ion as generated in polymerase chain reactions and pyrosequencing [J].
Anbu, Sellamuthu ;
Kamalraj, Subban ;
Paul, Anup ;
Jayabaskaran, Chelliah ;
Pombeiro, Armando J. L. .
DALTON TRANSACTIONS, 2015, 44 (09) :3930-3933
[2]  
[Anonymous], 2001, Biological Role of Inorganic Pyrophosphate
[3]   Nanomolar Pyrophosphate Detection in Water and in a Self-Assembled Hydrogel of a Simple Terpyridine-Zn2+ Complex [J].
Bhowmik, Sandip ;
Ghosh, Biswa Nath ;
Marjomaki, Varpu ;
Rissanen, Kari .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2014, 136 (15) :5543-5546
[4]   A red-emitting fluorescent probe for imaging hydrogen sulphide with a large Stokes shift [J].
Chen, Song ;
Hou, Peng ;
Song, Xiangzhi .
SENSORS AND ACTUATORS B-CHEMICAL, 2015, 221 :951-955
[5]   A Highly Selective Pyrophosphate Sensor Based on ESIPT Turn-On in Water [J].
Chen, Wei-Hua ;
Xing, Yu ;
Pang, Yi .
ORGANIC LETTERS, 2011, 13 (06) :1362-1365
[6]   Association between synovial fluid levels of inorganic pyrophosphate and short term radiographic outcome of knee osteoarthritis [J].
Doherty, M ;
Belcher, C ;
Regan, M ;
Jones, A ;
Ledingham, J .
ANNALS OF THE RHEUMATIC DISEASES, 1996, 55 (07) :432-436
[7]   A fluorescent probe for site I binding and sensitive discrimination of HSA from BSA [J].
Fan, Jiangli ;
Sun, Wen ;
Wang, Zhenkuan ;
Peng, Xiaojun ;
Li, Yueqing ;
Cao, Jianfang .
CHEMICAL COMMUNICATIONS, 2014, 50 (67) :9573-9576
[8]   Near-Infrared Fluorescent Turn-on Probe with a Remarkable Large Stokes Shift for Imaging Selenocysteine in Living Cells and Animals [J].
Feng, Weiyong ;
Li, Meixing ;
Sun, Yao ;
Feng, Guoqiang .
ANALYTICAL CHEMISTRY, 2017, 89 (11) :6107-6113
[9]   Fluorine NMR reporter for phosphate anions [J].
Gan, Haiying ;
Oliver, Allen G. ;
Smith, Bradley D. .
CHEMICAL COMMUNICATIONS, 2013, 49 (44) :5070-5072
[10]   Real-Time Tracking and In Vivo Visualization of β-Galactosidase Activity in Colorectal Tumor with a Ratiometric Near-Infrared Fluorescent Probe [J].
Gu, Kaizhi ;
Xu, Yisheng ;
Li, Hui ;
Guo, Zhiqian ;
Zhu, Shaojia ;
Zhu, Shiqin ;
Shi, Ping ;
James, Tony D. ;
Tian, He ;
Zhu, Wei-Hong .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2016, 138 (16) :5334-5340
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