Specific binding and self-structure induction to poly(A) by the cytotoxic plant alkaloid sanguinarine

被引：75

作者：

Giri, Prabal ^{[1
]}

Kumar, Gopinatha Suresh ^{[1
]}

机构：

[1] Indian Inst Chem Biol, Biophys Chem Lab, Kolkata 700032, W Bengal, India

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS | 2007年 / 1770卷 / 09期

关键词：

sanguinarine; poly(A); specific binding; self-structure formation; spectroscopy; thermodynamics;

D O I：

10.1016/j.bbagen.2007.05.005

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cytotoxic plant alkaloid sanguinarine was found to bind preferentially and strongly to single stranded poly(A) with an association constant (K-a) in the range 3.6-4.6 x 10(6) M(-1)in comparison to several nucleic acids. The binding induced unique self-structure formation in poly(A) that showed cooperative melting transition in circular dichroism, absorbance, and differential scanning calorimetry studies. The alkaloid binding was characterized to be intercalation as revealed from fluorescence quenching experiments and was predominantly enthalpy driven as revealed from isothermal titration calorimetry. Sanguinarine is the first and only natural product so far known to induce a self-structure formation in poly(A). (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：1419 / 1426

页数：8

共 47 条

[1]

Adhami VM, 2004, MOL CANCER THER, V3, P933

[2]

Ahmad N, 2000, CLIN CANCER RES, V6, P1524

[3] CD SPECTRAL COMPARISONS OF THE ACID-INDUCED STRUCTURES OF POLY[D(A)], POLY[R(A)], POLY[D(C)], AND POLY[R(C)] [J].

ANTAO, VP ;

GRAY, DM .

JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS, 1993, 10 (05) :819-839

[4] DNA-binding affinities and sequence selectivity of quaternary benzophenanthridine alkaloids sanguinarine, chelerythrine, and nitidine [J].

Bai, Li-Ping ;

Zhao, Zhong-Zhen ;

Cai, Zongwei ;

Jiang, Zhi-Hong .

BIOORGANIC & MEDICINAL CHEMISTRY, 2006, 14 (16) :5439-5445

[5]

Bajaj N P, 1990, J Mol Recognit, V3, P48, DOI 10.1002/jmr.300030106

[6] Protonated structures of naturally occurring deoxyribonucleic acids and their interaction with berberine [J].

Bhadra, K ;

Kumar, GS ;

Das, S ;

Islam, MM ;

Maiti, M .

BIOORGANIC & MEDICINAL CHEMISTRY, 2005, 13 (16) :4851-4863

[7] Molecular recognition of DNA by small molecules: AT base pair specific intercalative binding of cytotoxic plant alkaloid palmatine [J].

Bhadra, Kakah ;

Maiti, Motilal ;

Kumar, Gopinatha Suresh .

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 2007, 1770 (07) :1071-1080

[8]

Chaires Jonathan B., 2005, Current Medicinal Chemistry - Anti-Cancer Agents, V5, P339, DOI 10.2174/1568011054222292

[9] A thermodynamic signature for drug-DNA binding mode [J].

Chaires, Jonathan B. .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2006, 453 (01) :26-31

[10] Spectroscopic and thermodynamic studies on the binding of sanguinarine and berberine to triple and double helical DNA and RNA structures [J].

Das, S ;

Kumar, GS ;

Ray, A ;

Maiti, M .

JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS, 2003, 20 (05) :703-713

← 1 2 3 4 5 →