Inhibition of degranulation and cytokine production in bone marrow-derived mast cells by hydrolyzed rice bran

被引：11

作者：

Hoshino, Yuka ^{[2
]}

Hirashima, Naohide ^{[2
]}

Nakanishi, Mamoru ^{[1
]}

Furuno, Tadahide ^{[1
]}

机构：

[1] Aichi Gakuin Univ, Sch Pharm, Chikusa Ku, Nagoya, Aichi 4648650, Japan

[2] Nagoya City Univ, Grad Sch Pharmaceut Sci, Mizuho Ku, Nagoya, Aichi 4678603, Japan

来源：

INFLAMMATION RESEARCH | 2010年 / 59卷 / 08期

关键词：

Rice Bran; Mast Cell; Degranulation; Cytokine; Phosphorylation; FC-EPSILON RECEPTOR; KAPPA-B; RBL-2H3; CELLS; LEUKEMIA-CELLS; IN-VITRO; PROTEIN; MGN-3/BIOBRAN; ACTIVATION; EXOCYTOSIS; SNARES;

D O I：

10.1007/s00011-010-0173-9

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We investigated the effects of hydrolyzed rice bran (HRB), an arabinoxylan extracted from rice bran, on mast cell degranulation and cytokine production. HRB was obtained by treating rice bran with an extract obtained from shiitake mushrooms. Bone marrow-derived mast cells (BMMCs) were prepared by culturing bone marrow cells from BALB/c mice in the presence of interleukin-3 and stem cell factor for 4 weeks. BMMCs were pretreated with HRB (0-3 mg/ml) for 30 min and were then antigen activated. Pretreatment of BMMCs with HRB significantly inhibited antigen-induced degranulation and cytokine production (tumor necrosis factor-alpha and interleukin-4) in a dose-dependent manner. HRB also diminished membrane fusion between liposomes in which soluble N-ethyl maleimide-sensitive factor attachment protein receptors were reconstituted. Phosphorylation of RelA and mitogen-activated kinases after antigen stimulation was suppressed by pretreatment of BMMCs with HRB. These findings suggest that HRB may have an anti-inflammatory effect by inhibiting mast cell degranulation and cytokine production.

引用

页码：615 / 625

页数：11

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