Flow cytometric analyses of the viability, surface marker expression and function of lymphocytes from children following cryopreservation

被引：6

作者：

Chen, Xi ^{[1
,2
]}

Zhang, Hui ^{[1
,2
]}

Mou, Wenjun ^{[1
,2
]}

Qi, Zhan ^{[1
,3
]}

Ren, Xiaoya ^{[1
,4
]}

Wang, Guoliang ^{[1
,2
]}

Jiao, Hong ^{[1
,2
]}

Kong, Xiaohui ^{[1
,2
]}

Gui, Jingang ^{[1
,2
]}

机构：

[1] Capital Med Univ, Beijing Childrens Hosp, Key Lab Major Dis Children, Minist Educ, Beijing 100045, Peoples R China

[2] Capital Med Univ, Beijing Childrens Hosp, Lab Immunol, Beijing 100045, Peoples R China

[3] Capital Med Univ, Beijing Childrens Hosp, Beijing Pediat Res Inst, Ctr Med Genet, Beijing 100045, Peoples R China

[4] Capital Med Univ, Beijing Childrens Hosp, Dept Pediat Endocrinol & Genet Metab, Beijing 100045, Peoples R China

来源：

MOLECULAR MEDICINE REPORTS | 2016年 / 14卷 / 05期

关键词：

flow cytometry; children; lymphocytes; cryopreservation; cytokines; T-cell receptor repertoire; AGE-RELATED-CHANGES; PERIPHERAL-BLOOD; BETA; REPERTOIRE; RECEPTORS; ALPHA; SERUM;

D O I：

10.3892/mmr.2016.5780

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Flow cytometric analysis is important for the investigation and clinical preparation of lymphocytes from children. However, the strict requirement of cell freshness and inter-assay variability limits the application of this methodology for pediatric investigations. Therefore, it is necessary to identify a reliable cryopreservative method capable of maintaining high cell viability and proper cell function in lymphocytes from children. In the present study, eight commonly-used cell cyropreservative methods were used, and their effects on cell viability, surface marker expression and cell function were examined. In addition, how these methods affect the distribution of T-cell receptor V beta subfamilies were also determined. The results of the present study provided valuable experimental evidence, based on which the optimal method for the cryopreservation of lymphocytes from children in pediatric investigations and clinical applications can be selected.

引用

页码：4301 / 4308

页数：8

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