Requirement for Chloride Channel Function during the Hepatitis C Virus Life Cycle

被引：19

作者：

Igloi, Zsofia ^{[1
]}

Mohl, Bjorn-Patrick ^{[1
]}

Lippiat, Jonathan D. ^{[2
]}

Harris, Mark ^{[1
]}

Mankouri, Jamel ^{[1
]}

机构：

[1] Univ Leeds, Fac Biol Sci, Sch Mol & Cellular Biol, Leeds, W Yorkshire, England

[2] Univ Leeds, Fac Biol Sci, Sch Biomed Sci, Leeds, W Yorkshire, England

来源：

JOURNAL OF VIROLOGY | 2015年 / 89卷 / 07期

基金：

英国惠康基金;

关键词：

GENOTYPE; 1; ENDOSOMAL ACIDIFICATION; K+ CHANNEL; REPLICATION; INFECTION; COMPLEX; CULTURE; BLOCK;

D O I：

10.1128/JVI.02946-14

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Hepatocytes express an array of plasma membrane and intracellular ion channels, yet their role during the hepatitis C virus (HCV) life cycle remains largely undefined. Here, we show that HCV increases intracellular hepatic chloride (Cl-) influx that can be inhibited by selective Cl- channel blockers. Through pharmacological and small interfering RNA (siRNA)-mediated silencing, we demonstrate that Cl- channel inhibition is detrimental to HCV replication. This represents the first observation of the involvement of Cl- channels during the HCV life cycle.

引用

页码：4023 / 4029

页数：7

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