Selective determination of cysteine using BSA-stabilized gold nanoclusters with red emission

被引：72

作者：

Cui, Ma-Lin ^{[1
]}

Liu, Jia-Ming ^{[1
]}

Wang, Xin-Xing ^{[1
]}

Lin, Li-Ping ^{[1
]}

Jiao, Li ^{[1
]}

Zhang, Li-Hong ^{[2
]}

Zheng, Zhi-Yong ^{[2
]}

Lin, Shao-Qin ^{[3
]}

机构：

[1] Zhangzhou Normal Coll, Dept Chem & Environm Sci, Zhangzhou 363000, Peoples R China

[2] Zhangzhou Inst Technol, Dept Food & Biol Engn, Zhangzhou 363000, Peoples R China

[3] Fujian Educ Coll, Dept Biochem, Fuzhou 350001, Peoples R China

来源：

ANALYST | 2012年 / 137卷 / 22期

关键词：

PERFORMANCE LIQUID-CHROMATOGRAPHY; ELECTROCATALYTIC OXIDATION; HUMAN PLASMA; HOMOCYSTEINE; ASSAY; ACID; HG2+;

D O I：

10.1039/c2an36284h

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Gold nanoclusters (AuNCs) were synthesized by a macromolecules template using bovine serum albumin (BSA) as stabilizer which can emit red photoluminescence under illumination of ultraviolet light. The fluorescence intensity of AuNCs enhanced through decreasing the surface defects of AuNCs modified with cysteine, herein we present a novel fluorometry for determination of trace cysteine. This method with a wider linear range from 2.0 to 800 nmol mL(-1), higher sensitivity (detection limit was 1.2 nmol mL(-1)) and better selectivity has been utilized to determine cysteine content in real samples, and the results were in a good agreement with those determined by electrochemical biosensor. At the same time, the structures of AuNCs and AuNCs-cysteine were characterized by Fourier-transform infrared spectroscopy (FTIR) and high resolution transmission electron microscopy (HRTEM) and the mechanism of the proposed assay for the detection of cysteine has been discussed.

引用

页码：5346 / 5351

页数：6

共 32 条

[11] Using Gold Nanoclusters As Selective Luminescent Probes for Phosphate-Containing Metabolites [J].

Li, Po-Han ;

Lin, Ju-Yu ;

Chen, Cheng-Tai ;

Ciou, Wei-Ru ;

Chan, Po-Han ;

Luo, Liyang ;

Hsu, Hung-Yu ;

Diau, Eric Wei-Guang ;

Chen, Yu-Chie .

ANALYTICAL CHEMISTRY, 2012, 84 (13) :5484-5488

[12] A Ratiometric Fluorescent Probe for Cysteine and Homocysteine Displaying a Large Emission Shift [J].

Lin, Weiying ;

Long, Lingliang ;

Yuan, Lin ;

Cao, Zengmei ;

Chen, Bingbing ;

Tan, Wen .

ORGANIC LETTERS, 2008, 10 (24) :5577-5580

[13] Ultrasensitive Sensing of Hg2+ and CH3Hg+ Based on the Fluorescence Quenching of Lysozyme Type VI-Stabilized Gold Nanoclusters [J].

Lin, Yen-Hsiu ;

Tseng, Wei-Lung .

ANALYTICAL CHEMISTRY, 2010, 82 (22) :9194-9200

[14] Rapid sonochemical synthesis of highly luminescent non-toxic AuNCs and Au@AgNCs and Cu (II) sensing [J].

Liu, Hongying ;

Zhang, Xiang ;

Wu, Ximei ;

Jiang, Liping ;

Burda, Clemens ;

Zhu, Jun-Jie .

CHEMICAL COMMUNICATIONS, 2011, 47 (14) :4237-4239

[15]

Niu H, 2000, J CHEM TECHNOL BIOT, V75, P436, DOI 10.1002/1097-4660(200006)75:6<436::AID-JCTB243>3.0.CO

[16]

2-O

[17]

Pfeiffer CM, 1999, CLIN CHEM, V45, P290

[18] Molecular-receptor-specific, non-toxic, near-infrared-emitting Au cluster-protein nanoconjugates for targeted cancer imaging [J].

Retnakumari, Archana ;

Setua, Sonali ;

Menon, Deepthy ;

Ravindran, Prasanth ;

Muhammed, Habeeb ;

Pradeep, Thalappil ;

Nair, Shantikumar ;

Koyakutty, Manzoor .

NANOTECHNOLOGY, 2010, 21 (05)

[19] AMINO ACID COMPOSITION OF HUMAN HAIR [J].

ROBBINS, CR ;

KELLY, CH .

TEXTILE RESEARCH JOURNAL, 1970, 40 (10) :891-+

[20] Visual detection of cysteine and homocysteine [J].

Rusin, O ;

St Luce, NN ;

Agbaria, RA ;

Escobedo, JO ;

Jiang, S ;

Warner, IM ;

Dawan, FB ;

Lian, K ;

Strongin, RM .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (02) :438-439

← 1 2 3 4 →