Quantitative evaluation of the role of cysteine and methionine residues in the antioxidant activity of human serum albumin using recombinant mutants

被引：49

作者：

Iwao, Yasunori ^{[1
,2
]}

Ishima, Yu ^{[1
,3
]}

Yamada, Junji ^{[1
]}

Noguchi, Taishi ^{[1
]}

Kragh-Hansen, Ulrich ^{[4
]}

Mera, Katsumi ^{[1
]}

Honda, Daisuke ^{[1
]}

Suenaga, Ayaka ^{[1
]}

Maruyama, Toru ^{[1
,3
]}

Otagiri, Masaki ^{[1
,5
]}

机构：

[1] Kumamoto Univ, Dept Biopharmaceut, Grad Sch Pharmaceut Sci, Kumamoto 8620973, Japan

[2] Univ Shizuoka, Sch Pharmaceut Sci, Dept Pharmaceut Engn, Shizuoka 4228526, Japan

[3] Kumamoto Univ, Ctr Clin Pharmaceut Sci, Kumamoto, Japan

[4] Univ Aarhus, Dept Med Biochem, DK-8000 Aarhus C, Denmark

[5] Sojo Univ, Fac Pharmaceut Sci, Kumamoto, Japan

来源：

IUBMB LIFE | 2012年 / 64卷 / 05期

关键词：

human serum albumin; antioxidant activity; reactive oxygen species; nitric oxide; cysteine; methionine; OXIDATIVE STRESS; DISEASE; PROTEINS;

D O I：

10.1002/iub.567

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The importance of cysteine (Cys) and methionine (Met) residues for the antioxidant activity of human serum albumin (HSA) was investigated using recombinant HSA mutants, in which Cys34 and/or the six Met residues had been mutated to Ala. The scavenging activities of the mutants against five reactive oxygen and nitrogen species were evaluated by a chemiluminescence assay, electron paramagnetic resonance spectroscopy, or a HPLC-flow reactor assay. Our results showed that the contributions of Cys34 and the Met residues to the antioxidant activity of HSA were 61% and 29% against O2 -, 68% and 61% against H2O2, 38% and 6% against HO, 36% and 13% against HOCl, and 51% and 1% against NO, respectively. Thus, the findings propose in a direct way that Cys34 plays a more important role than the Met residues in the antioxidant activity of HSA. (c) 2012 IUBMB Life, 2012

引用

页码：450 / 454

页数：5

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