Quantitative evaluation of the role of cysteine and methionine residues in the antioxidant activity of human serum albumin using recombinant mutants

被引:49
作者
Iwao, Yasunori [1 ,2 ]
Ishima, Yu [1 ,3 ]
Yamada, Junji [1 ]
Noguchi, Taishi [1 ]
Kragh-Hansen, Ulrich [4 ]
Mera, Katsumi [1 ]
Honda, Daisuke [1 ]
Suenaga, Ayaka [1 ]
Maruyama, Toru [1 ,3 ]
Otagiri, Masaki [1 ,5 ]
机构
[1] Kumamoto Univ, Dept Biopharmaceut, Grad Sch Pharmaceut Sci, Kumamoto 8620973, Japan
[2] Univ Shizuoka, Sch Pharmaceut Sci, Dept Pharmaceut Engn, Shizuoka 4228526, Japan
[3] Kumamoto Univ, Ctr Clin Pharmaceut Sci, Kumamoto, Japan
[4] Univ Aarhus, Dept Med Biochem, DK-8000 Aarhus C, Denmark
[5] Sojo Univ, Fac Pharmaceut Sci, Kumamoto, Japan
关键词
human serum albumin; antioxidant activity; reactive oxygen species; nitric oxide; cysteine; methionine; OXIDATIVE STRESS; DISEASE; PROTEINS;
D O I
10.1002/iub.567
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The importance of cysteine (Cys) and methionine (Met) residues for the antioxidant activity of human serum albumin (HSA) was investigated using recombinant HSA mutants, in which Cys34 and/or the six Met residues had been mutated to Ala. The scavenging activities of the mutants against five reactive oxygen and nitrogen species were evaluated by a chemiluminescence assay, electron paramagnetic resonance spectroscopy, or a HPLC-flow reactor assay. Our results showed that the contributions of Cys34 and the Met residues to the antioxidant activity of HSA were 61% and 29% against O2 -, 68% and 61% against H2O2, 38% and 6% against HO, 36% and 13% against HOCl, and 51% and 1% against NO, respectively. Thus, the findings propose in a direct way that Cys34 plays a more important role than the Met residues in the antioxidant activity of HSA. (c) 2012 IUBMB Life, 2012
引用
收藏
页码:450 / 454
页数:5
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