Lysine detection and quantification by laser desorption/ionization mass spectrometry on gold nanoparticle-enhanced target

被引：20

作者：

Arendowski, Adrian ^{[1
]}

Ruman, Tomasz ^{[1
]}

机构：

[1] Rzeszow Univ Technol, Fac Chem, 6 Powstancow Warszawy Ave, PL-35959 Rzeszow, Poland

来源：

ANALYTICAL METHODS | 2018年 / 10卷 / 45期

关键词：

UNDERIVATIZED AMINO-ACIDS; LIQUID-CHROMATOGRAPHY; QUANTITATIVE-ANALYSIS; SMALL MOLECULES; MS; SENSITIVITY; IONIZATION; ARGININE; TISSUE; AUNPET;

D O I：

10.1039/c8ay01677a

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Laser desorption/ionization mass spectrometry method on gold nanoparticle enhanced target (AuNPET) was used for rapid detection and quantification of lysine. The amino acid was tested directly in the concentration range from 100 g mL(-1) to 1 ng mL(-1), which corresponds to lysine mass of 50 ng to 500 fg per analyzed spot. Detection limit value of 0.2 ng mL(-1) or 100 fg per spot is one of the best among the techniques used to determine lysine. In addition, mass spectrometry imaging quantification approach shown in this study canceled out sweet spot effect on the measurement result.

引用

页码：5398 / 5405

页数：8

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