Soluble Form of the (Pro) Renin Receptor Is Augmented in the Collecting Duct and Urine of Chronic Angiotensin II-Dependent Hypertensive Rats

被引：129
作者：
Gonzalez, Alexis A. [1 ,2 ]
Lara, Lucienne S. [1 ,2 ,4 ]
Luffman, Christina [1 ]
Seth, Dale M. [1 ]
Prieto, Minolfa C. [1 ,2 ,3 ]
机构：
[1] Tulane Univ, Sch Med, Dept Physiol, New Orleans, LA 70112 USA
[2] Tulane Univ, Sch Med, Tulane Hypertens & Renal Ctr, New Orleans, LA 70112 USA
[3] Tulane Univ, Sch Med, Tulane Bldg Interdisciplinary Res Careers Womens, New Orleans, LA 70112 USA
[4] Univ Fed Rio de Janeiro, Inst Ciencias Biomed, Rio De Janeiro, Brazil
来源：
HYPERTENSION | 2011年 / 57卷 / 04期
关键词：
Ang II-dependent hypertension; intrarenal renin-angiotensin system; intratubular RAS; collecting duct renin; (PRO)RENIN RECEPTOR; RENIN/PRORENIN RECEPTOR; GLOMERULOSCLEROSIS; PRORENIN; ENHANCEMENT; BIOLOGY; SYSTEM; FURIN;
D O I：
10.1161/HYPERTENSIONAHA.110.167957
中图分类号：
R6 [外科学];
学科分类号：
1002 ; 100210 ;
摘要：
Renin synthesis and secretion by principal cells of the collecting duct are enhanced in angiotensin (Ang) II-dependent hypertension. The presence of renin/(pro) renin and its receptor, the (pro) renin receptor ([P]RR), in the collecting duct may provide a pathway for Ang I generation with further conversion to Ang II. To assess whether (P) RR activation occurs during Ang II-dependent hypertension, we examined renal (P) RR levels and soluble (P) RR excretion in the urine of chronic Ang II-infused rats (80 ng/min; for 2 weeks; n=10) and sham-operated rats (n=10). Systolic blood pressure and Ang II levels in the plasma and kidney were increased whereas plasma renin activity was suppressed in Ang II-infused rats. Renal (P) RR transcripts were upregulated in the cortex and medulla of Ang II-infused rats. (P) RR immunoreactivity in collecting duct cells and the protein levels of the full-length form (37-kDa band) were significantly decreased in the medulla of Ang II-infused rats. The soluble (P) RR (28-kDa band) was detected in the renal medulla and urine samples of Ang II-infused rats, which also showed increases in urinary renin content. To determine whether the soluble (P) RR could stimulate Ang I formation, urine samples were incubated with recombinant human (pro) renin. Urine samples of Ang II-infused rats exhibited increased Ang I formation compared with sham-operated rats. Thus, in chronic Ang II-infused rats, the catalytic activity of the augmented renin produced in the collecting duct may be enhanced by the intraluminal soluble (P) RR and cell-surface located (P) RR, thus contributing to enhanced intratubular Ang II formation. (Hypertension. 2011;57:859-864.)
引用
收藏
页码：859 / 864
页数：6
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