Anti-Cancer Potential of Two Plasma-Activated Liquids: Implication of Long-Lived Reactive Oxygen and Nitrogen Species

被引:48
作者
Griseti, Elena [1 ,2 ]
Merbahi, Nofel [2 ]
Golzio, Muriel [1 ]
机构
[1] IPBS, Inst Pharmacol & Biol Struct, CNRS, UMR 5089, 205 Route Narbonne, Toulouse 31077, France
[2] Univ Toulouse III Paul Sabatier, LAPLACE, Lab Plasmas & Convers Energie, CNRS,UMR 5213, 118 Route Narbonne Bat,3R3, Toulouse 31062, France
关键词
cancer; plasma-activated liquids; multicellular tumor spheroids; long-lived reactive oxygen and nitrogen species; COLD ATMOSPHERIC PLASMA; CANCER-CELLS; MECHANISMS;
D O I
10.3390/cancers12030721
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Cold atmospheric plasma-exposed culture medium may efficiently kill cancer cells in vitro. Due to the complexity of the medium obtained after plasma exposure, less complex physiological liquids, such as saline solutions and saline buffers, are gathering momentum. Among the plethora of reactive oxygen and nitrogen species (RONS) that are produced in these plasma-activated liquids, hydrogen peroxide, nitrite and nitrate appear to be mainly responsible for cytotoxic and genotoxic effects. Here, we evaluated the anti-cancer potential of plasma-activated phosphate-buffered saline (P-A PBS) and sodium chloride 0.9% (P-A NaCl), using a three-dimensional tumor model. Two epithelial cancer cell lines were used to evaluate cellular effects of either P-A PBS or P-A NaCl. Human colorectal cancer cells HCT 116 and human ovarian carcinoma, SKOV-3 were used to investigate the manner by which different cell types respond to different plasma-activated liquids treatments. Our investigations indicate that P-A PBS is more efficient than P-A NaCl mainly because RONS are produced in larger quantities. Indeed, we show that the cytotoxicity of these liquids directly correlates with the concentration of hydrogen peroxide and nitrite. Moreover, P-A PBS induced a faster-occurring and more pronounced cell death, which arose within deeper layers of the 3D multicellular spheroid models.
引用
收藏
页数:15
相关论文
共 32 条
[1]   Targeting Cancer Cells with Reactive Oxygen and Nitrogen Species Generated by Atmospheric-Pressure Air Plasma [J].
Ahn, Hak Jun ;
Kim, Kang Il ;
Nguyen Ngoc Hoan ;
Kim, Churl Ho ;
Moon, Eunpyo ;
Choi, Kyeong Sook ;
Yang, Sang Sik ;
Lee, Jong-Soo .
PLOS ONE, 2014, 9 (01)
[2]   Plasma-activated medium triggers cell death and the presentation of immune activating danger signals in melanoma and pancreatic cancer cells [J].
Azzariti, Amalie ;
Iacobazzi, Rosa Maria ;
Di Fonte, Roberta ;
Porcelli, Letizia ;
Gristina, Roberto ;
Favia, Pietro ;
Fracassi, Francesco ;
Trizio, Ilaria ;
Silvestris, Nicola ;
Guida, Gabriella ;
Tommasi, Stefanie ;
Sardella, Eloisa .
SCIENTIFIC REPORTS, 2019, 9 (1)
[3]   Signal Amplification by Tumor Cells: Clue to the Understanding of the Antitumor Effects of Cold Atmospheric Plasma and Plasma-Activated Medium [J].
Bauer, Georg .
IEEE TRANSACTIONS ON RADIATION AND PLASMA MEDICAL SCIENCES, 2018, 2 (02) :87-98
[4]   Mechanisms of Selective Antitumor Action of Cold Atmospheric Plasma-Derived Reactive Oxygen and Nitrogen Species [J].
Bauer, Georg ;
Graves, David B. .
PLASMA PROCESSES AND POLYMERS, 2016, 13 (12) :1157-1178
[5]   The Influence of Cell Type and Culture Medium on Determining Cancer Selectivity of Cold Atmospheric Plasma Treatment [J].
Biscop, Eline ;
Lin, Abraham ;
Van Boxem, Wilma ;
Van Loenhout, Jinthe ;
De Backer, Joey ;
Deben, Christophe ;
Dewilde, Sylvia ;
Smits, Evelien ;
Bogaerts, Annemie .
CANCERS, 2019, 11 (09)
[6]   Elucidation of in vitro cellular steps induced by antitumor treatment with plasma-activated medium [J].
Chauvin, Julie ;
Gibot, Laure ;
Griseti, Elena ;
Golzio, Muriel ;
Rols, Marie-Pierre ;
Merbahi, Nofel ;
Vicendo, Patricia .
SCIENTIFIC REPORTS, 2019, 9 (1)
[7]   Analysis of reactive oxygen and nitrogen species generated in three liquid media by low temperature helium plasma jet [J].
Chauvin, Julie ;
Judee, Florian ;
Yousfi, Mohammed ;
Vicendo, Patricia ;
Merbahi, Nofel .
SCIENTIFIC REPORTS, 2017, 7
[8]   In Vitro Anticancer Efficacy of Six Different Clinically Approved Types of Liquids Exposed to Physical Plasma [J].
Freund, Eric ;
Liedtke, Kim Rouven ;
Gebbe, Rebecca ;
Heidecke, Amanda Kathleen ;
Partecke, Lars-Ivo ;
Bekeschus, Sander .
IEEE TRANSACTIONS ON RADIATION AND PLASMA MEDICAL SCIENCES, 2019, 3 (05) :588-596
[9]   Synergistic Effect of H2O2 and NO2 in Cell Death Induced by Cold Atmospheric He Plasma [J].
Girard, Pierre-Marie ;
Arbabian, Atousa ;
Fleury, Michel ;
Bauville, Gerard ;
Puech, Vincent ;
Dutreix, Marie ;
Sousa, Joao Santos .
SCIENTIFIC REPORTS, 2016, 6
[10]   Analysis of Short-Lived Reactive Species in Plasma-Air-Water Systems: The Dos and the Do Nots [J].
Gorbanev, Yury ;
Privat-Maldonado, Angela ;
Bogaerts, Annemie .
ANALYTICAL CHEMISTRY, 2018, 90 (22) :13151-13158