Simultaneous determination of icariin, icariside II and osthole in rat plasma after oral administration of the extract of Gushudan (a Chinese compound formulation) by LC-MS/MS

被引：48

作者：

Liu, Man ^{[1
]}

Liu, Huiping ^{[1
]}

Lu, Xiumei ^{[1
]}

Li, Chao ^{[1
]}

Xiong, Zhili ^{[1
]}

Li, Famei ^{[1
]}

机构：

[1] Shenyang Pharmaceut Univ, Dept Analyt Chem, Shenyang 110016, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2007年 / 860卷 / 01期

基金：

中国国家自然科学基金;

关键词：

icariin; icariside II; osthole; Gushudan; LC-MS/MS; pharmacokinetics;

D O I：

10.1016/j.jchromb.2007.10.019

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A sensitive, specific and accurate LC-MS/MS method was developed for simultaneous determination of icariin, icariside II and osthole in rat plasma. With carbamazepine as the internal standard, plasma samples were prepared by protein precipitation with acetonitrile. Analysis was carried out on an ACQUITY UPLC (TM) BEH C-18 column with a linear gradient and 0.1% aqueous acetic acid and acetonitrile were used as mobile phase. Detection was performed by means of electrospray ionization mass spectrometry in positive ion mode with multiple reaction monitoring. Linear calibration curves of icariin, icariside 11 and osthole were obtained over the concentration ranges of 2.00-200, 2.00-200 and 2.00-500 ng/ml, respectively. The intra-and inter-day precisions were within 8.0% and 14%, and the accuracy was from -6.0% to 9.0%. The method was successfully applied to pharmacokinetic studies of icariin, icariside II and osthole in rats after oral administration of Gushudan extract. (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：113 / 120

页数：8

共 17 条

[11]

Qiu Feng, 1999, Acta Pharmaceutica Sinica, V34, P222

[12]

THAI TH, 1996, J PHARM BIOMED ANAL, V14, P749

[13]

[王婷 WANG Ting], 2006, [中草药, Chinese Traditional and Herbal Drugs], V37, P1458

[14]

XIAO QB, 2005, SCI TECHNOLOG MED SC, V25, P690

[15]

Xu, 2006, WORLD SCI TECHNOL MO, V8, P98

[16]

Ye L., 1999, CHIN PHARM J, V34, P33

[17]

Zhang Y Q, 2000, ACAD J SEC MIL MED U, V21, P935

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