New Criterion for Evaluation of Honey: Quantification of Royal Jelly Protein Apalbumin 1 in Honey by ELISA

被引:43
作者
Bilikova, Katarina [1 ]
Simuth, Jozef [1 ]
机构
[1] Slovak Acad Sci, Inst Mol Biol, Dept Mol Apidol, Bratislava 84551, Slovakia
关键词
Honey; apalbumin1; royal jelly; ELISA; adulteration; TNF-ALPHA RELEASE; APIS-MELLIFERA; HYPOPHARYNGEAL GLAND; PURIFICATION; GLUCOSIDASE; EXPRESSION; COMPONENT; PEPTIDE; FAMILY;
D O I
10.1021/jf101583s
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
The 55 kDa major protein of royal jelly, named apalbumin 1, is an authentic protein of honey and pollen pellet, and for its quantification an enzyme-linked immunosorbent assay (ELISA) was developed using specific polyclonal anti-apalbumin 1 antibody. The limit of detection for apalbumin 1 was 2 ng mL(-1). The floral honeys contained apalbumin 1 as follows: acacia, 0.011%; linden, 0.010%; chestnut, 0.029%; rape, 0.010%; and dandelion, 0.014%. The saccharose syrup honey contained only 0.001% of apalbumin 1. The average amount of apalbumin 1 relating to the total protein content of analyzed honey samples was 23.39%, whereas in SCCH apalbumin 1 presented only 4.81% of total proteins of honey. Apalbumin 1 is thermostabile in honey at 80 degrees C incubated for 40 min. ELISA results show good precision in the evaluation of apalbumin 1 quantity in honey (CV ranged from 0.69 to 4.25%).
引用
收藏
页码:8776 / 8781
页数:6
相关论文
共 29 条
[1]   A review of the analytical methods to determine the geographical and botanical origin of honey [J].
Anklam, E .
FOOD CHEMISTRY, 1998, 63 (04) :549-562
[2]   Protein contents and physicochemical properties in honey samples of Apis mellifera of different floral origins [J].
Azeredo, LD ;
Azeredo, MAA ;
de Souza, SR ;
Dutra, VML .
FOOD CHEMISTRY, 2003, 80 (02) :249-254
[3]   Honey amylase activity and food starch degradation [J].
Babacan, S ;
Pivarnik, LF ;
Rand, AG .
JOURNAL OF FOOD SCIENCE, 2002, 67 (05) :1625-1630
[4]  
BARBERAN T, 2001, J SCI FOOD AGR, V81, P485
[5]   Development of a competitive ELISA for the evaluation of sunflower pollen in honey samples [J].
Baroni, MV ;
Chiabrando, GA ;
Costa, C ;
Fagúndez, GA ;
Wunderlin, DA .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2004, 52 (24) :7222-7226
[6]   Apisimin, a new serine-valine-rich peptide from honeybee (Apis mellifera L.) royal jelly:: purification and molecular characterization [J].
Bíliková, K ;
Hanes, J ;
Nordhoff, E ;
Saenger, W ;
Klaudiny, J ;
Simúth, J .
FEBS LETTERS, 2002, 528 (1-3) :125-129
[7]  
Bíliková K, 1999, BIOLOGIA, V54, P733
[8]  
Bíliková K, 2001, APIDOLOGIE, V32, P275, DOI 10.1051/apido:2001129
[9]   Towards functional proteomics of minority component of honeybee royal jelly: The effect of post-translational modifications on the antimicrobial activity of apalbumin2 [J].
Bilikova, Katarina ;
Mirgorodskaya, Ekaterina ;
Bukovska, Gabriela ;
Gobom, Johan ;
Lehrach, Hans ;
Simuth, Jozef .
PROTEOMICS, 2009, 9 (08) :2131-2138
[10]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3