Role of cytokines in promoting immune escape of FasL-expressing human colon cancer cells

被引:0
|
作者
Xu, Tong [1 ]
Sun, Bao-Cun [2 ]
Li, Qiang [1 ]
Hao, Xi-Shan [1 ]
机构
[1] Tianjin Med Univ, Dept Abdominal Surg, Canc Hosp, Tianjin 300060, Peoples R China
[2] Tianjin Med Univ, Inst Oncol, Tianjin 300060, Peoples R China
关键词
Cytokines; Fas/Fas ligand; Colon cancer; Apoptosis; Immune escape;
D O I
暂无
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
AIM: To investigate the potential role of cytokines in promoting Fas ligand (FasL)-expressing colon cancer cells. METHODS: Immunohistochemical SABC method was used to observe the expression of Fas receptor and ligand in SW620 colon cancer cell line and Jurkat T cells in order to provide the morphological evidence for the functions of Fas receptor and ligand. To examine the cytotoxicity of effector cells, CytoTox96 (R) non-radioactive cytotoxicity assay was adopted to measure the lactate dehydrogenase-releasing value after SW620 cells were co-cultured with Jurkat T lymphocytes. RESULTS: The FasL of colon cancer SW620 cells was positive. The positive substances were distributed in the cell membrane and cytoplasm. The Fas receptor of colon cancer SW620 cells was negative. The Fas receptor and ligand of Jurkat T lymphocytes turned out to be positive. The positive substances were distributed in the cell membrane. After phytohemagglutinin (PHA)-stimulated Jurkat T lymphocytes were co-cultured with phorbol 12-myristate 13-acetate (PMA)-plus-ionomycin-stimulated (for 48 h) SW620 cells or tumor necrosis factor-alpha (TNF-alpha)-stimulated (for 48 h) SW620 cells or unstimulated SW620 cells for 4 h, the cytotoxicity of SW620 cells to PHA-stimulated Jurkat cells at effector-to-target ratios of 10: 1, 5: 1, 2.5:1, and 1.25:1 was 74.6%, 40.8%, 32.4%, and 10.9% (F = 8.19, P<0.05); or 54.9%, 35.3%, 22.0%, and 10.3% (F = 11.12, P<0.05); or 14.9%, 10.5%, 6.9%, and 5.8% (F = 3.45, P<0.05). After PHA-stimulated Jurkat T lymphocytes were co-cultured with unstimulated SW620 cells for 8 h, the cytotoxicity of SW620 cells to PHA-stimulated Jurkat cells at effector-to-target ratios of 5: 1, 2.5: 1, and 1.25: 1 from the experiment was 83.9%, 74.1%, and 28.5% (F = 137.04, P<0.05) respectively. Non-radioactive cytotoxicity assay showed that the apoptotic rate of Jurkat cells remarkably increased with the increase of planting concentration of SW620 cells and co-culture time after the SW620 cells were co-cultured with the Jurkat T lymphocytes. The cytotoxicity was significantly enhanced by PMA+ionomycin or TNF-alpha. CONCLUSION: The FasL expressed in human colon cancer cells may be regulated by endogenous factors in the microenvironment of the host and facilitate the escape of tumor cells from the host immune system. (C) 2005 The WJG Press and Elsevier Inc. All rights reserved.
引用
收藏
页码:3915 / 3919
页数:5
相关论文
共 50 条
  • [31] RETRACTED: A Role for PPARγ in the Regulation of Cytokines in Immune Cells and Cancer (Retracted Article)
    Yang, Xiao Yi
    Wang, Li Hua
    Farrar, William L.
    PPAR RESEARCH, 2008, 2008
  • [32] The role of immune cytokines in the human inflammatory pulp
    TaniIshii, N
    Kamakura, H
    Watanabe, Y
    Umemoto, T
    DENTIN/PULP COMPLEX, 1996, : 329 - 330
  • [33] Role of cytokines and chemokines for cancer immune prevention
    Haabeth, Ole Audun Werner
    Lorvik, Kristina Berg
    Aagaard, Lise
    Bogen, Bjarne
    Corthay, Alexandre
    SCANDINAVIAN JOURNAL OF IMMUNOLOGY, 2008, 68 (02) : 218 - 218
  • [34] The role of cancer-derived microRNAs in cancer immune escape
    Yi, Ming
    Xu, Linping
    Jiao, Ying
    Luo, Suxia
    Li, Anping
    Wu, Kongming
    JOURNAL OF HEMATOLOGY & ONCOLOGY, 2020, 13 (01)
  • [35] The role of cancer-derived microRNAs in cancer immune escape
    Ming Yi
    Linping Xu
    Ying Jiao
    Suxia Luo
    Anping Li
    Kongming Wu
    Journal of Hematology & Oncology, 13
  • [36] Role of mitochondrial function in the invasiveness of human colon cancer cells
    Lin, Chen-Sung
    Liu, Li-Tzu
    Ou, Liang-Hung
    Pan, Siao-Cian
    Lin, Chia-I
    Wei, Yau-Huei
    ONCOLOGY REPORTS, 2018, 39 (01) : 316 - 330
  • [37] Correlation Between Immune Lymphoid Cells and Plasmacytoid Dendritic Cells in Human Colon Cancer
    Wu, Jing
    Cheng, Hang
    Wang, Helei
    Zang, Guoxia
    Qi, Lingli
    Lv, Xinping
    Liu, Chunyan
    Zhu, Shan
    Zhang, Mingyou
    Cui, Jiuwei
    Ueno, Hideki
    Liu, Yong-Jun
    Suo, Jian
    Chen, Jingtao
    FRONTIERS IN IMMUNOLOGY, 2021, 12
  • [38] Dual role of pseudogene TMEM198B in promoting lipid metabolism and immune escape of glioma cells
    Zhan, Ying
    Qiao, Wei
    Yi, Bolong
    Yang, Xinyu
    Li, Miaomiao
    Sun, Lu
    Ji, Lian
    Su, Peng
    Wang, Xin
    Zhang, Furong
    Zhang, Rui
    Gao, Mingjun
    Zhao, Wujun
    Song, Yichen
    ONCOGENE, 2022, 41 (40) : 4512 - 4523
  • [39] Dual role of pseudogene TMEM198B in promoting lipid metabolism and immune escape of glioma cells
    Ying Zhan
    Wei Qiao
    Bolong Yi
    Xinyu Yang
    Miaomiao Li
    Lu Sun
    Lian Ji
    Peng Su
    Xin Wang
    Furong Zhang
    Rui Zhang
    Mingjun Gao
    Wujun Zhao
    Yichen Song
    Oncogene, 2022, 41 : 4512 - 4523
  • [40] Dendritic cells and cytokines in immune rejection of cancer
    Ferrantini, Maria
    Capone, Imerio
    Belardelli, Filippo
    CYTOKINE & GROWTH FACTOR REVIEWS, 2008, 19 (01) : 93 - 107