Validation of LUCIO®-Direct-ELISA kits for the detection of drugs of abuse in urine: Application to the new German driving licence re-granting guidelines

被引：19

作者：

Agius, Ronald ^{[1
]}

Nadulski, Thomas ^{[1
]}

Moore, Christine ^{[2
]}

机构：

[1] Lab Krone, D-32105 Bad Salzuflen, Germany

[2] Immunalysis Corp, Pomona, CA 91767 USA

来源：

FORENSIC SCIENCE INTERNATIONAL | 2012年 / 215卷 / 1-3期

关键词：

Drug screening; Zero-tolerance cut-offs; Driving licence re-granting guidelines; Abstinence control; Uncertainty of measurement; Forensic accreditation; STABILITY; AMPHETAMINE; SPECIMENS; MORPHINE; MS;

D O I：

10.1016/j.forsciint.2011.10.034

中图分类号：

DF [法律]; D9 [法律]; R [医药、卫生];

学科分类号：

0301 ; 10 ;

摘要：

LUCIO (R)-Direct-enzyme linked immunosorbent assay (ELISA) tests were validated for the screening of drugs of abuse cannabis, opiates, amphetamines and cocaine in urine for the new German medical and psychological assessment (MPA) guidelines with subsequent gas chromatographic-mass spectrometric (GC-MS) confirmation. The screening cut-offs corresponding to 10 ng/mL 11-nor-delta-9-tetrahydrocannabinol-9-carboxylic acid (THC-COOH), 50 ng/mL amphetamine, 25 ng/mL morphine and codeine and 30 ng/mL benzoylecgonine were chosen at the point where the number of false negatives was lower than 1%. Due to their accuracy, ease of use and rapid analysis, these ELISA tests are very promising for cases where a large proportion of the tests are expected to be negative such as for abstinence monitoring as part of the driving licence re-granting process. (C) 2011 Elsevier Ireland Ltd. All rights reserved.

引用

页码：38 / 45

页数：8

共 21 条

[1] Simultaneous GC-El-MS determination of Δ9-tetrahydrocannabinol, 11-hydroxy-Δ9-tetrahydrocannabinol, and 11-nor-9-carboxy, Δ9-tetrahydrocannabinol in human urine following tandem enzyme-alkaline hydrolysis [J].