Quantitative analysis of Alaska pollock in seafood products by droplet digital PCR

被引：20

作者：

Noh, Eun Soo ^{[1
]}

Park, Yeon Jung ^{[1
]}

Kim, Eun Mi ^{[1
]}

Park, Jung Youn ^{[1
]}

Shim, Kil Bo ^{[2
]}

Choi, Tae-Jin ^{[3
]}

Kim, Kyoung-Ho ^{[3
]}

Kang, Jung-Ha ^{[1
]}

机构：

[1] Natl Inst Fisheries Sci, Biotechnol Res Div, 216 Gijanghaean Ro, Busan 46083, South Korea

[2] Natl Inst Fisheries Sci, Food Safety & Proc Res Div, 216 Gijanghaean Ro, Busan 46083, South Korea

[3] Pukyong Natl Univ, Dept Microbiol, Coll Nat Sci, 45 Yongso Ro, Busan 48513, South Korea

来源：

FOOD CHEMISTRY | 2019年 / 275卷

关键词：

Droplet digital PCR; Quantitative analysis; Alaska pollock; Seafood products; REAL-TIME PCR; SPECIES IDENTIFICATION; DNA; FISH; QUANTIFICATION; SUBSTITUTION; MARKET;

D O I：

10.1016/j.foodchem.2018.09.093

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A highly accurate quantitative method, based on the new technique, droplet digital PCR (ddPCR), was applied to determine the content of Alaska pollock (Gadus chalcogrammus) in seafood products. Using this method, we found a linear relationship among raw sample weight, DNA concentration and DNA copy number. We also established a formula to calculate the raw sample weight, based on the number of DNA copies. To confirm the accuracy and applicability of this method, mixed samples of known composition were analyzed. Results from this study indicated that the ddPCR method described is suitable for quantifying Alaska pollock in seafood products and has the potential applied to a variety of tasks in food quality certification.

引用

页码：638 / 643

页数：6

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