Nanoformulations of a potent copper-based aquaporin inhibitor with cytotoxic effect against cancer cells

被引:49
作者
Nave, Mariana [1 ]
Castro, Rui E. [1 ]
Rodrigues, Cecilia M. P. [1 ]
Casini, Angela [2 ]
Soveral, Graca [1 ]
Manuela Gaspar, Maria [1 ]
机构
[1] Univ Lisbon, Res Inst Med iMed ULisboa, Fac Pharm, Av Prof Gama Pinto, P-1649003 Lisbon, Portugal
[2] Cardiff Univ, Sch Chem, Pk Pl, Cardiff CF10 3AT, S Glam, Wales
关键词
aquaporins; colon cancer; Cuphen; cytotoxic effect; in vivo toxicity studies; liposomes; melanoma; metallodrugs; INDUCED DOWN-REGULATION; BIOLOGICAL CHARACTERIZATION; TARGETED DELIVERY; GOLD COMPOUNDS; L-ASPARAGINASE; DRUG-DELIVERY; IN-VITRO; COMPLEXES; LIPOSOMES; APOPTOSIS;
D O I
10.2217/nnm-2016-0086
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Aim: Development of liposomal formulations of Cuphen, a potent copper-based aquaporin inhibitor with therapeutic potential against melanoma and colon cancer. Materials & methods: Cuphen was incorporated into liposomes using the dehydration-rehydration method. The ability of Cuphen to induce cancer cell death was evaluated by MTS and ViaCount assays. In vivo toxicity studies were performed in BALB/c mice. Results: In vitro studies illustrated the antiproliferative effects of Cuphen in different cancer cell lines, in free form or after incorporation into liposomes. In vivo studies revealed no toxic effects after parenteral administration of Cuphen liposomes. Conclusions: Cuphen liposomes are highly attractive to be further tested in murine models due to the possibility of stabilizing and specifically deliver this metallodrug to tumor sites.
引用
收藏
页码:1817 / 1830
页数:14
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