Association of protein kinase c μ with type II phosphatidylinositol 4-kinase and type I phosphatidylinositol-4-phosphate 5-kinase

被引:76
|
作者
Nishikawa, K
Toker, A
Wong, K
Marignani, PA
Johannes, FJ
Cantley, LC
机构
[1] Harvard Univ, Sch Med, Div Signal Transduct, Beth Israel Deaconess Med Ctr, Boston, MA 02115 USA
[2] Harvard Univ, Sch Med, Dept Cell Biol, Boston, MA 02115 USA
[3] Boston Biomed Res Inst, Boston, MA 02114 USA
[4] Univ Stuttgart, Inst Cell Biol & Immunol, D-70569 Stuttgart, Germany
关键词
D O I
10.1074/jbc.273.36.23126
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Protein kinase C mu (PKC mu), also named protein kinase D, is an unusual member of the PKC family that has a putative transmembrane domain and pleckstrin homology domain. This enzyme has a substrate specificity distinct from other PKC isoforms (Nishikawa, K., Toker, A., Johannes, F. J., Songyang, Z., and Cantley, L. C. (1997) J. Biol. Chem. 272, 952-960), and its mechanism of regulation is not yet clear. Here we show that PKC mu forms a complex in vivo with a phosphatidylinositol 4-kinase and a phosphatidylinositol-4-phosphate 5-kinase. A region of PKC mu between the amino-terminal transmembrane domain and the pleckstrin homology domain is shown to be involved in the association with the lipid kinases. Interestingly, a kinase-dead point mutant of PKC mu. failed to associate with either lipid kinase activity, indicating that autophosphorylation may be required to expose the Lipid kinase interaction domain. Furthermore, the subcellular distribution of the PKC mu-associated lipid kinases to the particulate fraction depends on the presence of the amino-terminal. region of PKC mu including the predicted transmembrane region. These results suggest a novel model in which the noncatalytic region of PKC mu acts as a scaffold for assembly of enzymes involved in phosphoinositide synthesis at specific membrane locations.
引用
收藏
页码:23126 / 23133
页数:8
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