Studies on Chromatographic Fractioning by Cations Exchangers of a Bovine Hemoglobin Hydrolysate

被引：0

作者：

Panainte, Alina Diana ^{[1
]}

Morariu, Ionela Daniela ^{[1
]}

Bibire, Nela ^{[1
]}

Vieriu, Madalina ^{[1
]}

Tantaru, Gladiola ^{[1
]}

Luca, Alina Costina ^{[2
]}

Apostu, Mihai ^{[1
]}

机构：

[1] Grigore T Popa Univ Med & Pharm, Fac Pharm, 16th Univ Str, Iasi 700115, Romania

[2] Grigore T Popa Univ Med & Pharm, Fac Med, 16th Univ Str, Iasi 700115, Romania

来源：

REVISTA DE CHIMIE | 2018年 / 69卷 / 10期

关键词：

hemoglobin; cation exchangers; RP-HPLC; ANTIBACTERIAL ACTIVITY; BIOACTIVE PEPTIDES; SEPARATION; BLOOD;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A peptidic hydrolysate has been obtained through hydrolysis of bovine hemoglobin using pepsin. The fractioning of the hydrolysate was performed on a column packed with CM-Sepharose Fast Flow. The hydrolysate and each fraction was filtered and then injected into a HPLC system equipped with a Vydak C4 reverse phase column (0.46 x 25 cm), suitable for the chromatographic separation of large peptides with 20 to 30 amino adds. The detection was done using mass spectrometry, and the retention time, size and distribution of the peptides were determined.

引用

页码：2794 / 2798

页数：5

共 50 条

[41] Synthesis, characterization and photoluminescence of substituted terpyridine compounds and their molecular docking studies with bovine hemoglobin
Zhou, Peng
Huang, Ling
Zhang, Yongqiang
Xue, Xingyong
Zhou, Yanling
Ma, Zhen
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY, 2018, 358 : 17 - 25
[42] Studies on the interaction between neutral red and bovine hemoglobin by fluorescence spectroscopy and molecular modeling
Wang, Ruiqiang
Li, Zhigang
Yang, Lingling
Ren, Ting
Zhang, Lijiao
Wang, Ruiyong
JOURNAL OF MOLECULAR LIQUIDS, 2015, 211 : 584 - 590
[43] STUDIES ON ION-EXCHANGE RESINS .4. SELECTIVITY COEFFICIENTS OF VARIOUS CATION EXCHANGERS TOWARDS UNIVALENT CATIONS
GREGOR, HP
BREGMAN, JI
JOURNAL OF COLLOID SCIENCE, 1951, 6 (04): : 323 - 347
[44] STUDIES ON HEMOGLOBIN .6. AMINO ACID COMPOSITIONS OF FRACTIONATED BOVINE GLOBIN ALPHA AND BETA
SASAKAWA, S
JOURNAL OF BIOCHEMISTRY, 1961, 50 (04): : 345 - &
[45] STUDIES ON HISTONES FROM JUVENILE AND ADULT BOVINE OVARIAN TISSUES - TOTAL YIELD AND CHROMATOGRAPHIC FRACTIONATION
SINGER, R
BER, A
INTERNATIONAL JOURNAL OF BIOCHEMISTRY, 1972, 3 (18): : 642 - 646
[46] STUDIES ON ERYTHROCYTE HEMINS .4. RELEASE OF HEMINS FROM HEMOGLOBIN ON THIN-LAYER CHROMATOGRAPHIC PLATE
MIYAUCHI, C
KATAGIRI, E
OKADA, T
PROCEEDINGS OF THE JAPAN ACADEMY, 1969, 45 (09): : 820 - &
[47] STUDIES ON HEMOGLOBIN .3. ARGININE-PEPTIDES IN THE TRYPTIC AND CHYMOTRYPTIC HYDROLYSATES OF BOVINE AND HORSE GLOBINS
OZAWA, H
TAMAI, K
SATAKE, K
JOURNAL OF BIOCHEMISTRY, 1960, 47 (02): : 244 - 252
[48] Unraveling the binding interaction between polyvinyl chloride microplastics and bovine hemoglobin: Multi-spectroscopic studies
Wang, Xin
Li, Haoyang
Li, Dong
He, Yanping
Zhang, Sanjun
Chen, Jinquan
Xu, Jianhua
JOURNAL OF MOLECULAR STRUCTURE, 2022, 1269
[49] STUDIES ON HETEROGENEITY OF HEMOGLOBIN .8. CHROMATOGRAPHIC AND ELECTROPHORETIC INVESTIGATIONS OF VARIOUS MINOR HEMOGLOBIN FRACTIONS PRESENT IN NORMAL AND IN VITRO MODIFIED RED BLOOD CELL HEMOLYSATES
HUISMAN, THJ
HORTON, BF
JOURNAL OF CHROMATOGRAPHY, 1965, 18 (01): : 116 - &
[50] Effect of Chromatographic Conditions on Enantioseparation of Bovine Serum Albumin Chiral Stationary Phase in HPLC and Thermodynamic Studies
Wang, Qiu-Yun
Xiong, Ya-Jin
Lu, Bao-Zhu
Fan, Jun
Zheng, Sheng-Run
Zhang, Wei-Guang
CHIRALITY, 2013, 25 (09) : 487 - 492

← 1 2 3 4 5 →