Studies on Chromatographic Fractioning by Cations Exchangers of a Bovine Hemoglobin Hydrolysate

被引：0

作者：

Panainte, Alina Diana ^{[1
]}

Morariu, Ionela Daniela ^{[1
]}

Bibire, Nela ^{[1
]}

Vieriu, Madalina ^{[1
]}

Tantaru, Gladiola ^{[1
]}

Luca, Alina Costina ^{[2
]}

Apostu, Mihai ^{[1
]}

机构：

[1] Grigore T Popa Univ Med & Pharm, Fac Pharm, 16th Univ Str, Iasi 700115, Romania

[2] Grigore T Popa Univ Med & Pharm, Fac Med, 16th Univ Str, Iasi 700115, Romania

来源：

REVISTA DE CHIMIE | 2018年 / 69卷 / 10期

关键词：

hemoglobin; cation exchangers; RP-HPLC; ANTIBACTERIAL ACTIVITY; BIOACTIVE PEPTIDES; SEPARATION; BLOOD;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A peptidic hydrolysate has been obtained through hydrolysis of bovine hemoglobin using pepsin. The fractioning of the hydrolysate was performed on a column packed with CM-Sepharose Fast Flow. The hydrolysate and each fraction was filtered and then injected into a HPLC system equipped with a Vydak C4 reverse phase column (0.46 x 25 cm), suitable for the chromatographic separation of large peptides with 20 to 30 amino adds. The detection was done using mass spectrometry, and the retention time, size and distribution of the peptides were determined.

引用

页码：2794 / 2798

页数：5

共 50 条

[1] STUDIES ON HEMOGLOBIN .5. CHROMATOGRAPHIC RESOLUTION OF BOVINE GLOBIN INTO VALYLLEUCYL AND METHIONYL CHAINS
SASAKAWA, S
JOURNAL OF BIOCHEMISTRY, 1961, 49 (03): : 200 - &
[2] Influence of molecular interactions on ultrafiltration of a bovine hemoglobin hydrolysate with an organic membrane
Lebrun, F
Bazus, A
Dhulster, P
Guillochon, D
JOURNAL OF MEMBRANE SCIENCE, 1998, 146 (01) : 113 - 124
[3] Influence of molecular interactions on ultrafiltration of a bovine hemoglobin hydrolysate with an organic membrane
Lab. Technol. Substances Naturelles, IUTA Lille I, BP 179, 59653 Villeneuve d'Ascq Cedex, France
J. Membr. Sci., 1 (113-124):
[4] Studies on the interaction of caffeine with bovine hemoglobin
Wang, Yan-Qing
Zhang, Hong-Mei
Zhou, Qiu-Hua
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY, 2009, 44 (05) : 2100 - 2105
[5] Identification of hemorphins in a cathepsin D bovine hemoglobin hydrolysate by radioimmunoassay and photodiode array detections
Garreau, I
Fruitier, I
Sannier, F
Zhao, QY
Cucumel, K
Cupo, A
Piot, JM
LETTERS IN PEPTIDE SCIENCE, 1997, 4 (4-6): : 293 - 296
[6] ISOLATION AND CHARACTERIZATION OF 2 OPIOID-PEPTIDES FROM A BOVINE HEMOGLOBIN PEPTIC HYDROLYSATE
PIOT, JM
ZHAO, Q
GUILLOCHON, D
RICART, G
THOMAS, D
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1992, 189 (01) : 101 - 110
[7] Identification of hemorphins in a cathepsin D bovine hemoglobin hydrolysate by radioimmunoassay and photodiode array detections
Isabelle Garreau
Ingrid Fruitier
Frederic Sannier
Qiuyu Zhao
Karine Cucumel
Anny Cupo
Jean-Marie Piot
Letters in Peptide Science, 1997, 4 : 293 - 296
[8] Identification of hemorphins in a cathepsin D bovine hemoglobin hydrolysate by radioimmunoassay and photodiode array detections
Isabelle Garreau
Ingrid Fruitier
Frederic Sannier
Qiuyu Zhao
Karine Cucumel
Anny Cupo
Jean-Marie Piot
Letters in Peptide Science, 1997, 4 : 293 - 296
[9] ISOLATION AND CHARACTERIZATION OF A BRADYKININ-POTENTIATING PEPTIDE FROM A BOVINE PEPTIC HEMOGLOBIN HYDROLYSATE
PIOT, JM
ZHAO, QY
GUILLOCHON, D
RICART, G
THOMAS, D
FEBS LETTERS, 1992, 299 (01) : 75 - 79
[10] Studies on the interaction of palmatine hydrochloride with bovine hemoglobin
Liu, Baosheng
Yan, Xiaona
Cao, Shina
Chong, Baohong
Lu, Yunkai
LUMINESCENCE, 2014, 29 (03) : 211 - 218

← 1 2 3 4 5 →