Modeling of the influence of the experimental conditions on the separation of the components of a binary mixture in isocratic overloaded elution preparative chromatography

The elution band profiles of the components of a binary mixture were calculated for systematic variations of the experimental conditions. Three values of the separation factor were used, 1.8, 1.3 and 1.1. The column efficiency was 3000 theoretical plates, except for alpha = 1.1, in which case efficiencies of both 3000 and 5000 plates were used, The relative compositions of 3:1, 1:1, and 1:3 were selected. The values of the loading factor were chosen to illustrate the increasing degrees of interference and band interaction which take place with increasing column loading. In each case, the cutting volumes for the production of each component at a 98% purity were calculated and tabulated. The influence of the experimental conditions on the recovery yield is discussed.