High frequency gene conversion among benomyl resistant transformants in the entomopathogenic fungus Metarhizium anisopliae

被引：18
作者：
Bogo, MR
Vainstein, MH
Aragao, FJL
Rech, E
Schrank, A
机构：
[1] UNIV FED RIO GRANDE SUL,DEPT BIOTECNOL,CTR BIOTECNOL,BR-90540000 PORTO ALEGRE,RS,BRAZIL
[2] UNIV BRASILIA,DEPT BIOL CELULAR,BR-70849970 BRASILIA,DF,BRAZIL
[3] EMBRAPA,CENARGEN,BR-70849970 BRASILIA,DF,BRAZIL
来源：
FEMS MICROBIOLOGY LETTERS | 1996年 / 142卷 / 01期
关键词：
Metarhizium anisopliae; fungal transformation; electroporation; biolistic; gene conversion; benomyl resistance;
D O I：
10.1111/j.1574-6968.1996.tb08418.x
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Three different methods, (i) PEG, (ii) electroporation and (iii) biolistic, were employed to transform Metarhizium anisopliae using benomyl resistance as a selectable marker. Transformation frequencies and mitotic stability varied for each method, from 0.8 to 6.9 transformants mu g(-1) of DNA and 46%, respectively, by the PEG method; 1.3 to 1.8 transformants mu g(-1) of DNA and 67% by electroporation; and 32 to 201 transformants mu g(-1) of DNA and 90% by biolistic. We demonstrate by PCR that 60% of the transformants were generated by gene conversion.
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页码：123 / 127
页数：5
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