Biotransformation of withanolides by cell suspension cultures of Withania somnifera (Dunal)

被引：18

作者：

Sabir, Farzana ^{[1
]}

Sangwan, Rajender S. ^{[1
]}

Singh, Jyoti ^{[1
]}

Misra, Laxmi N. ^{[1
]}

Pathak, Neelam ^{[2
]}

Sangwan, Neelam S. ^{[1
]}

机构：

[1] CSIR, CIMAP, Lucknow 226015, Uttar Pradesh, India

[2] Integral Univ, Lucknow, Uttar Pradesh, India

来源：

PLANT BIOTECHNOLOGY REPORTS | 2011年 / 5卷 / 02期

关键词：

Ashwagandha; Biotransformation; Cell suspension cultures; Withanolide; Withania somnifera; ROOTS; INHIBITION;

D O I：

10.1007/s11816-011-0165-4

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The biotransformation potential of cell suspension cultures generated from Withania somnifera leaf was investigated, using withanolides, i.e. withanolide A, withaferin A, and withanone as precursor substrates. Interestingly, the cell suspension cultures showed inter-conversion of withanolides, as well converted to some unknown compounds, released to the culture media. The bio-catalyzed withanolide was detected and quantified by TLC and HPLC, respectively. There is noticeable conversion of withanolide A to withanone, and vice versa though at a lower level. The type of reaction of this biotransformation appears to be substitution of 20-OH group to 17-OH in withanolide A. In this paper, we present for the first time the possibility of biotransformation by inter-conversion of withanolides of pharmacological importance through cell suspension culture of W. somnifera. The possible role of putative cytochrome P-450 hydroxylases is implicated in the conversion.

引用

页码：127 / 134

页数：8

共 19 条

[1]

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[2] Chlorinated and diepoxy withanolides from Withania somnifera and their cytotoxic effects against human lung cancer cell line [J].