Rapid determination of chloramphenicol residues in aquaculture tissues by immunochromatographic assay

被引：27
作者：
Li, Ke [1 ]
Liu, Liqiang
Xu, ChuanLai
Chu, XiaoGang
机构：
[1] WuXi kangBaiEr Biotechnol Corp, Wuxi 214011, JiangSu, Peoples R China
[2] So Yangtze Univ, Sch Food Sci & Technol, WuXi 214036, JiangSu, Peoples R China
[3] Chinese Acad Inspect & Quarantine, Beijing 100082, Peoples R China
来源：
ANALYTICAL SCIENCES | 2007年 / 23卷 / 11期
关键词：
D O I：
10.2116/analsci.23.1281
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
An immunochromatographic assay was developed to detect chloramphenicol (CAP) residues in aquaculture tissues. The limit of detection (LOD) was 10 ng g(-1) for detecting CAP spiked in the aquaculture tissues. The results were confirmed by liquid chromatography tandem mass spectrometry (LC/MS/MS) and indicated that there was a good agreement between the two methods. The linear regression equation was y = 1.19x + 0.539 with R-2 = 0.978. The assay time for test was less than 5 min and the method is suitable for rapid testing on-site.
引用
收藏
页码：1281 / 1284
页数：4
相关论文
共 20 条
  • [1] DOT-ELISA FOR THE RAPID DETECTION OF GENTAMICIN IN MILK
    ARA, J
    GANS, Z
    SWEENEY, R
    WOLF, B
    [J]. JOURNAL OF CLINICAL LABORATORY ANALYSIS, 1995, 9 (05) : 320 - 324
  • [2] Chemiluminescence enzyme immunoassay (CLEIA) for the determination of chloramphenicol residues in aquatic tissues
    Chuanlai, X
    Peng, CF
    Hao, K
    Jin, ZY
    Wang, WK
    [J]. LUMINESCENCE, 2006, 21 (02) : 126 - 128
  • [3] HYBRIDOMA CELL-LINE PRODUCTION OF A SPECIFIC MONOCLONAL-ANTIBODY TO THE MYCOTOXINS ZEARALENONE AND ALPHA-ZEARALENOL
    DIXON, DE
    WARNER, RL
    RAM, BP
    HART, LP
    PESTKA, JJ
    [J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1987, 35 (01) : 122 - 126
  • [4] HARLOW ED, 1988, COLD SPRING HARBOR L, P196
  • [5] HEITZMAN RJ, 1994, VET DRUG RESIDUES, P127
  • [6] HUANG SL, 1991, ACTA AGR NUCL SINICA, V12, P291
  • [7] ENZYME IMMUNOASSAYS OF KANAMYCIN GROUP ANTIBIOTICS WITH HIGH SENSITIVITIES USING ANTI-KANAMYCIN AS A COMMON ANTI-SERUM - REASONING AND SELECTION OF A HETEROLOGOUS ENZYME LABEL
    KITAGAWA, T
    FUJIWARA, K
    TOMONOH, S
    TAKAHASHI, K
    KOIDA, M
    [J]. JOURNAL OF BIOCHEMISTRY, 1983, 94 (04) : 1165 - 1172
  • [8] Competitive ELISA of chloramphenicol: Influence of immunoreagent structure and application of the method for the inspection of food of animal origin
    Kolosova, AY
    Samsonova, JV
    Egorov, AM
    [J]. FOOD AND AGRICULTURAL IMMUNOLOGY, 2000, 12 (02) : 115 - 125
  • [9] Enzyme immunoassay using peroxidase as a label and a dip-strip test for monitoring residual bacitracin in chicken plasma
    Matsumoto, M
    Tsunematsu, K
    Tsuji, A
    Kido, Y
    [J]. ANALYTICA CHIMICA ACTA, 1997, 346 (02) : 207 - 213
  • [10] SIMULTANEOUS DETERMINATION OF THIAMPHENICOL, FLORFENICOL, AND CHLORAMPHENICOL RESIDUES IN MUSCLES OF ANIMALS AND CULTURED FISH BY LIQUID-CHROMATOGRAPHY
    NAGATA, T
    SAEKI, M
    [J]. JOURNAL OF LIQUID CHROMATOGRAPHY, 1992, 15 (12): : 2045 - 2056
12