Two-photon photostimulation and imaging of neural circuits

被引：194
|
作者：
Nikolenko, Volodymyr [1 ]
Poskanzer, Kira E. [1 ]
Yuste, Rafael [1 ]
机构：
[1] Columbia Univ, Dept Biol Sci, Howard Hughes Med Inst, New York, NY 10027 USA
来源：
NATURE METHODS | 2007年 / 4卷 / 11期
关键词：
D O I：
10.1038/NMETH1105
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We introduce an optical method to stimulate individual neurons in brain slices in any arbitrary spatiotemporal pattern, using two- photon uncaging of MNI-glutamate with beam multiplexing. This method has single-cell and three-dimensional precision. By sequentially stimulating up to a thousand potential presynaptic neurons, we generated detailed functional maps of inputs to a cell. We combined this approach with two- photon calcium imaging in an all-optical method to image and manipulate circuit activity.
引用
收藏
页码：943 / 950
页数：8
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