Two-photon photostimulation and imaging of neural circuits

被引：194

作者：

Nikolenko, Volodymyr ^{[1
]}

Poskanzer, Kira E. ^{[1
]}

Yuste, Rafael ^{[1
]}

机构：

[1] Columbia Univ, Dept Biol Sci, Howard Hughes Med Inst, New York, NY 10027 USA

来源：

NATURE METHODS | 2007年 / 4卷 / 11期

关键词：

D O I：

10.1038/NMETH1105

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We introduce an optical method to stimulate individual neurons in brain slices in any arbitrary spatiotemporal pattern, using two- photon uncaging of MNI-glutamate with beam multiplexing. This method has single-cell and three-dimensional precision. By sequentially stimulating up to a thousand potential presynaptic neurons, we generated detailed functional maps of inputs to a cell. We combined this approach with two- photon calcium imaging in an all-optical method to image and manipulate circuit activity.

引用

页码：943 / 950

页数：8

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