Enhanced sucrose fermentation by introduction of heterologous sucrose transporter and invertase into Clostridium beijerinckii for acetone-butanol-ethanol production

被引：2

作者：

Lin, Lihua ^{[1
,2
]}

Zhang, Zhikai ^{[1
]}

Tang, Hongchi ^{[1
,2
]}

Guo, Yuan ^{[2
]}

Zhou, Bingqing ^{[2
]}

Liu, Yi ^{[2
]}

Huang, Ribo ^{[1
]}

Du, Liqin ^{[1
]}

Pang, Hao ^{[2
]}

机构：

[1] Guangxi Univ, Coll Life Sci & Technol, Guangxi Res Ctr Microbial & Enzymat Technol, State Key Lab Conservat & Utilizat Subtrop Agrobi, Daxue Rd 100, Nanning 530005, Guangxi, Peoples R China

[2] Guangxi Acad Sci, Natl Engn Res Ctr Nonfood Biorefinery, Guangxi Key Lab Biorefinery, State Key Lab Nonfood Biomass & Enzyme Technol, Daling Rd 98, Nanning 530007, Guangxi, Peoples R China

来源：

ROYAL SOCIETY OPEN SCIENCE | 2021年 / 8卷 / 09期

关键词：

transporter; membrane protein; sucrose metabolism; microbial engineering; butanol; Clostridium beijerinckii; ACETOBUTYLICUM; METABOLISM; EXPRESSION;

D O I：

10.1098/rsos.201858

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A heterologous pathway for sucrose transport and metabolism was introduced into Clostridium beijerinckii to improve sucrose use for n-butanol production. The combined expression of StSUT1, encoding a sucrose transporter from potato (Solanum tuberosum), and SUC2, encoding a sucrose invertase from Saccharomyces cerevisiae, remarkably enhanced n-butanol production. With sucrose, sugarcane molasses and sugarcane juice as substrates, the C. beijerinckii strain harbouring StSUT1 and SUC2 increased acetone-butanol-ethanol production by 38.7%, 22.3% and 52.8%, respectively, compared with the wild-type strain. This is the first report to demonstrate enhanced sucrose fermentation due to the heterologous expression of a sucrose transporter and invertase in Clostridium. The metabolic engineering strategy used in this study can be widely applied in other microorganisms to enhance the production of high-value compounds from sucrose-based biomass.

引用

页数：9

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