Protein-based chiral stationary phases for high-performance liquid chromatography enantioseparations

被引：189

作者：

Haginaka, J ^{[1
]}

机构：

[1] Mukogawa Womens Univ, Fac Pharmaceut Sci, Nishinomiya, Hyogo 6638179, Japan

来源：

JOURNAL OF CHROMATOGRAPHY A | 2001年 / 906卷 / 1-2期

关键词：

reviews; chiral stationary phases; LC; enantiomer separation; proteins; glycoproteins; enzymes;

D O I：

10.1016/S0021-9673(00)00504-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The enantioseparations of various compounds using proteins as the chiral selectors in high-performance liquid chromatography (HPLC) are considered in this review. The proteins used include albumins such as bovine serum albumin and human serum albumin, glycoproteins such as alpha (1)-acid glycoprotein, ovomucoid, ovoglycoprotein, avidin and riboflavin binding protein, enzymes such as trypsin, alpha -chymotrypsin, cellobiohydrolase I, lysozyme, pepsin and amyloglucosidase, and other proteins such as ovotransferrin and beta -lactoglobulin. This review deals with the properties of HPLC chiral stationary phases based on proteins, and the enantioselective properties and chiral recognition mechanisms of these stationary phases. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：253 / 273

页数：21

共 111 条

[1] DIRECT LIQUID-CHROMATOGRAPHIC SEPARATION OF ENANTIOMERS ON IMMOBILIZED PROTEIN STATIONARY PHASES .4. MOLECULAR INTERACTION FORCES AND RETENTION BEHAVIOR IN CHROMATOGRAPHY ON BOVINE SERUM-ALBUMIN AS A STATIONARY PHASE [J].