Quantitative phase microscopy of biological samples using a portable interferometer

被引：186

作者：

Shaked, Natan T. ^{[1
]}

机构：

[1] Tel Aviv Univ, Fac Engn, Dept Biomed Engn, IL-69978 Tel Aviv, Israel

来源：

OPTICS LETTERS | 2012年 / 37卷 / 11期

关键词：

D O I：

10.1364/OL.37.002016

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

This Letter presents the tau interferometer, a portable and inexpensive device for obtaining spatial interferograms of microscopic biological samples without the strict stability and the highly coherent illumination that are usually required for interferometric microscopy setups. The device is built using off-the-shelf optical elements and can easily operate with low-coherence illumination, while being positioned in the output of a conventional inverted microscope. The interferograms are processed into the quantitative amplitude and phase profiles of the sample. Based on the phase profile, the optical-path-delay profile is obtained with temporal stability of 0.18 nm and spatial stability of 0.42 nm. Further experimental demonstration of using the tau interferometer for imaging the quantitative thickness profile of a live red blood cell is provided. (C) 2012 Optical Society of America

引用

页码：2016 / 2018

页数：3

共 15 条

[1] Quadriwave lateral shearing interferometry for quantitative phase microscopy of living cells [J].

Bon, Pierre ;

Maucort, Guillaume ;

Wattellier, Benoit ;

Monneret, Serge .

OPTICS EXPRESS, 2009, 17 (15) :13080-13094

[2] Total aberrations compensation in digital holographic microscopy with a reference conjugated hologram [J].

Colomb, T ;

Kühn, JK ;

Charrière, F ;

Depeursinge, C ;

Marquet, P ;

Aspert, N .

OPTICS EXPRESS, 2006, 14 (10) :4300-4306

[3] Digital self-referencing quantitative phase microscopy by wavefront folding in holographic image reconstruction [J].

Coppola, G. ;

Di Caprio, G. ;

Gioffre, M. ;

Puglisi, R. ;

Balduzzi, D. ;

Galli, A. ;

Miccio, L. ;

Paturzo, M. ;

Grilli, S. ;

Finizio, A. ;

Ferraro, P. .

OPTICS LETTERS, 2010, 35 (20) :3390-3392

[4] Wavefront image sensor chip [J].

Cui, Xiquan ;

Ren, Jian ;

Tearney, Guillermo J. ;

Yang, Changhuei .

OPTICS EXPRESS, 2010, 18 (16) :16685-16701

[5] Instantaneous spatial light interference microscopy [J].

Ding, Huafeng ;

Popescu, Gabriel .

OPTICS EXPRESS, 2010, 18 (02) :1569-1575

[6] Self-reference quantitative phase microscopy for microfluidic devices [J].

Jang, Jaeduck ;

Bae, Chae Yun ;

Park, Je-Kyun ;

Ye, Jong Chul .

OPTICS LETTERS, 2010, 35 (04) :514-516

[7] Simplified approach for quantitative digital holographic phase contrast imaging of living cells [J].

Kemper, Bjoern ;

Vollmer, Angelika ;

Rommel, Christina E. ;

Schnekenburger, Juergen ;

von Bally, Gert .

JOURNAL OF BIOMEDICAL OPTICS, 2011, 16 (02)

[8] Field-portable reflection and transmission microscopy based on lensless holography [J].

Lee, Myungjun ;

Yaglidere, Oguzhan ;

Ozcan, Aydogan .

BIOMEDICAL OPTICS EXPRESS, 2011, 2 (09) :2721-2730

[9] Common-path phase-shifting digital holographic microscopy:: A way to quantitative phase imaging and superresolution [J].

Mico, Vicente ;

Zalevsky, Zeev ;

Garcia, Javier .

OPTICS COMMUNICATIONS, 2008, 281 (17) :4273-4281

[10] Dual wavelength full field imaging in low coherence digital holographic microscopy [J].

Monemhaghdoust, Zahra ;

Montfort, Frederic ;

Emery, Yves ;

Depeursinge, Christian ;

Moser, Christophe .

OPTICS EXPRESS, 2011, 19 (24) :24005-24022

← 1 2 →