Persistence and genetic adaptation of Pseudomonas aeruginosa in patients with chronic obstructive pulmonary disease

被引：18

作者：

Eklof, Josefin ^{[1
,2
]}

Misiakou, Maria A. ^{[3
]}

Sivapalan, Pradeesh ^{[1
,2
]}

Armbruster, Karin ^{[1
,2
]}

Browatzki, Andrea ^{[4
,5
]}

Nielsen, Thyge L. ^{[4
,5
]}

Lapperre, Therese S. ^{[6
]}

Andreassen, Helle F. ^{[6
]}

Janner, Julie ^{[7
]}

Ulrik, Charlotte S. ^{[7
]}

Gabrielaite, Migle ^{[3
]}

Johansen, Helle K. ^{[8
,9
]}

Jensen, Annemette ^{[10
,11
]}

Nielsen, Tine, V ^{[10
,11
]}

Hertz, Frederik B. ^{[10
,11
]}

Ghathian, Khaled ^{[12
]}

Calum, Henrik ^{[12
]}

Wilcke, Torgny ^{[1
,2
]}

Seersholm, Niels ^{[1
,2
]}

Jensen, Jens-Ulrik S. ^{[1
,2
,9
]}

Marvig, Rasmus L. ^{[3
]}

机构：

[1] Copenhagen Univ Hosp, Dept Internal Med, Resp Med Sect, Herlev & Gentofte Hosp, Herlev, Denmark

[2] Copenhagen Univ Hosp, Dept Internal Med, Resp Med Sect, Herlev & Gentofte Hosp, Gentofte, Denmark

[3] Copenhagen Univ Hosp, Ctr Genom Med, Rigshosp, Copenhagen, Denmark

[4] Copenhagen Univ Hosp, Dept Resp & Infect Dis, North Zealand Hosp, Frederikssund, Denmark

[5] Copenhagen Univ Hosp, Dept Resp & Infect Dis, North Zealand Hosp, Hillerod, Denmark

[6] Copenhagen Univ Hosp, Dept Resp Med, Bispebjerg Hosp, Copenhagen, Denmark

[7] Copenhagen Univ Hosp, Dept Resp Med, Amager & Hvidovre Hosp, Hvidovre, Denmark

[8] Copenhagen Univ Hosp, Dept Clin Microbiol, Rigshosp, Copenhagen, Denmark

[9] Univ Copenhagen, Fac Hlth & Med Sci, Dept Clin Med, Copenhagen, Denmark

[10] Copenhagen Univ Hosp, Dept Clin Microbiol, Herlev & Gentofte Hosp, Herlev, Denmark

[11] Copenhagen Univ Hosp, Dept Clin Microbiol, Herlev & Gentofte Hosp, Gentofte, Denmark

[12] Copenhagen Univ Hosp, Dept Clin Microbiol, Amager & Hvidovre Hosp, Hvidovre, Denmark

来源：

CLINICAL MICROBIOLOGY AND INFECTION | 2022年 / 28卷 / 07期

基金：

新加坡国家研究基金会;

关键词：

Chronic obstructive pulmonary disease; Genetic adaptation; Pseudomonas aeruginosa; Whole-genome sequencing; CYSTIC-FIBROSIS; ANTIMICROBIAL RESISTANCE; EXACERBATION; INFECTION; EVOLUTION; STRAINS;

D O I：

10.1016/j.cmi.2022.01.017

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Objectives: It is unclear whether recurrent sputum culture with Pseudomonas aeruginosa from patients with chronic obstructive pulmonary disease (COPD) is caused by intermittent airway carriage by different P. aeruginosa lineages or persistent carriage by the same lineage, and whether lineages genetically adapt during carriage. Methods: Whole-genome sequencing was performed for P. aeruginosa isolates sampled longitudinally from sputum cultures in patients with COPD who were enrolled in an ongoing randomized controlled trial (clinicaltrials.gov: NCT03262142). Results: A total of 153 P. aeruginosa isolates were sequenced for 23 patients during 365 days of follow-up. Recurrent presence of P. aeruginosa was seen in 19 patients (83%) and was caused by persistence of the same clonal lineage in all but one patient. We identified 38 genes mutated in parallel in two or more lineages, suggesting positive selection for adaptive mutations. Mutational enrichment analysis revealed genes important in antibiotic resistance and chronic infections to be more frequently mutated. Discussion: Recurrent P. aeruginosa was common and carried for a prolonged time after initial detection in the airways of patients with COPD. Recurrence was caused by persistence of the same clonal lineage and was associated with genetic adaptation. Trial data on possible clinical benefits of attempting antibiotic eradication of P. aeruginosa in COPD are warranted. (C) 2022 The Author(s). Published by Elsevier Ltd on behalf of European Society of Clinical Microbiology and Infectious Diseases.

引用

页码：990 / 995

页数：6

共 30 条

[11] Sputum bacteriology in hospitalized patients with acute exacerbation of chronic obstructive pulmonary disease in Taiwan with an emphasis on Klebsiella pneumoniae and Pseudomonas aeruginosa [J].

Lin, Sheng-Hsiang ;

Kuo, Ping-Hung ;

Hsueh, Po-Ren ;

Yang, Pan-Chyr ;

Kuo, Sow-Hsong .

RESPIROLOGY, 2007, 12 (01) :81-87

[12] Evolution of the Pseudomonas aeruginosa mutational resistome in an international Cystic Fibrosis clone [J].

Lopez-Causape, Carla ;

Sommer, Lea Mette ;

Cabot, Gabriel ;

Rubio, Rosa ;

Ocampo-Sosa, Alain A. ;

Johansen, Helle Krogh ;

Figuerola, Joan ;

Canton, Rafael ;

Kidd, Timothy J. ;

Molin, Soeren ;

Oliver, Antonio .

SCIENTIFIC REPORTS, 2017, 7

[13] Hypermutation is a key factor in development of multiple-antimicrobial resistance in Pseudomonas aeruginosa strains causing chronic lung infections [J].

Maciá, MD ;

Blanquer, D ;

Togores, B ;

Sauleda, J ;

Pérez, JL ;

Oliver, A .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2005, 49 (08) :3382-3386

[14] Random amplified polymorphic DNA typing of Pseudomonas aeruginosa isolates recovered from patients with cystic fibrosis [J].

Mahenthiralingam, E ;

Campbell, ME ;

Foster, J ;

Lam, JS ;

Speert, DP .

JOURNAL OF CLINICAL MICROBIOLOGY, 1996, 34 (05) :1129-1135

[15] Chronic Pseudomonas aeruginosa Infection in Chronic Obstructive Pulmonary Disease [J].

Martinez-Solano, Laura ;

Macia, Maria D. ;

Fajardo, Alicia ;

Oliver, Antonio ;

Martinez, Jose L. .

CLINICAL INFECTIOUS DISEASES, 2008, 47 (12) :1526-1533

[16] Within-host microevolution of Pseudomonas aeruginosa in Italian cystic fibrosis patients [J].

Marvig, Rasmus Lykke ;

Dolce, Daniela ;

Sommer, Lea M. ;

Petersen, Bent ;

Ciofu, Oana ;

Campana, Silvia ;

Molin, Soren ;

Taccetti, Giovanni ;

Johansen, Helle Krogh .

BMC MICROBIOLOGY, 2015, 15

[17]

Marvig RL, 2015, FUTURE MICROBIOL, V10, P599, DOI [10.2217/fmb.15.3, 10.2217/FMB.15.3]

[18] Convergent evolution and adaptation of Pseudomonas aeruginosa within patients with cystic fibrosis [J].

Marvig, Rasmus Lykke ;

Sommer, Lea Mette ;

Molin, Soren ;

Johansen, Helle Krogh .

NATURE GENETICS, 2015, 47 (01) :57-+

[19] Genome Analysis of a Transmissible Lineage of Pseudomonas aeruginosa Reveals Pathoadaptive Mutations and Distinct Evolutionary Paths of Hypermutators [J].

Marvig, Rasmus Lykke ;

Johansen, Helle Krogh ;

Molin, Soren ;

Jelsbak, Lars .

PLOS GENETICS, 2013, 9 (09)

[20] Relationship between bacterial flora in sputum and functional impairment in patients with acute exacerbations of COPD [J].

Miravitlles, M ;

Espinosa, C ;

Fernández-Laso, E ;

Martos, JA ;

Maldonado, JA ;

Gallego, M .

CHEST, 1999, 116 (01) :40-46

← 1 2 3 →