Using a bifunctional polymer for the functionalization of Fe3O4 nanoparticles

被引：16
作者：
Zhang, Quan [2 ]
Luan, Liang [2 ]
Feng, Siliang [2 ]
Yan, Husheng [1 ]
Liu, Keliang [2 ]
机构：
[1] Nankai Univ, Key Lab Funct Polymer Mat, Minist Educ, Inst Polymer Chem, Tianjin 300071, Peoples R China
[2] Beijing Inst Pharmacol & Toxicol, Beijing 100850, Peoples R China
来源：
REACTIVE & FUNCTIONAL POLYMERS | 2012年 / 72卷 / 03期
基金：
中国国家自然科学基金;
关键词：
Bifunctional polymer; Functionalization of nanoparticles; Fe3O4; Nuclear delivery; Tat peptide; IRON-OXIDE NANOPARTICLES; MAGNETIC-RESONANCE DETECTION; SUPERPARAMAGNETIC NANOPARTICLES; DRUG-DELIVERY; TAT PEPTIDE; ANNEXIN-V; CELLS; PROTEIN; SURFACE; NANOCRYSTALS;
D O I：
10.1016/j.reactfunctpolym.2012.01.003
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
A bifunctional maleimido-tetra(ethylene glycol)-poly(glycerol monoacrylate) (MAL-TEG-PGA) polymer was synthesized and used as a linker to couple functional biomolecules to iron oxide nanoparticles. The cell-penetrating peptide Tat was chosen as a model ligand and successfully conjugated to the surface of Fe3O4 nanoparticles using MAL-TEG-PGA. The Tat-conjugated Fe3O4 nanoparticles can be prepared simply by applying the linker to the iron oxide nanoparticles and then coupling the Tat peptide to the maleimide terminus or by coating the nanoparticles with a pre-coupled linker. Cell-uptake studies demonstrated that the Tat peptide was an efficient functional biomolecule to translocate iron oxide nanopartides into the cell nucleus. Tat-conjugated nanoparticles thus prepared may be useful for drug or gene delivery. (C) 2012 Elsevier Ltd. All rights reserved.
引用
收藏
页码：198 / 205
页数：8
相关论文
共 40 条
[11]   Multifunctional nanostructured materials for multimodal imaging, and simultaneous imaging and therapy [J].
Kim, Jaeyun ;
Piao, Yuanzhe ;
Hyeon, Taeghwan .
CHEMICAL SOCIETY REVIEWS, 2009, 38 (02) :372-390
[12]   Uptake and metabolism of a dual fluorochrome tat-nanoparticle in HeLa cells [J].
Koch, AM ;
Reynolds, F ;
Kircher, MF ;
Merkle, HP ;
Weissleder, R ;
Josephson, L .
BIOCONJUGATE CHEMISTRY, 2003, 14 (06) :1115-1121
[13]   Magnetic resonance of a dextran-coated magnetic fluid intravenously administered in mice [J].
Lacava, LM ;
Lacava, ZGM ;
Da Silva, MF ;
Silva, O ;
Chaves, SB ;
Azevedo, RB ;
Pelegrini, F ;
Gansau, C ;
Buske, N ;
Sabolovic, D ;
Morais, PC .
BIOPHYSICAL JOURNAL, 2001, 80 (05) :2483-2486
[14]   Magnetic iron oxide nanoparticles: Synthesis, stabilization, vectorization, physicochemical characterizations, and biological applications [J].
Laurent, Sophie ;
Forge, Delphine ;
Port, Marc ;
Roch, Alain ;
Robic, Caroline ;
Elst, Luce Vander ;
Muller, Robert N. .
CHEMICAL REVIEWS, 2008, 108 (06) :2064-2110
[15]   Functionalization Strategies for Protease Immobilization on Magnetic Nanoparticles [J].
Li, Dan ;
Teoh, Wey Yang ;
Gooding, J. Justin ;
Selomulya, Cordelia ;
Amal, Rose .
ADVANCED FUNCTIONAL MATERIALS, 2010, 20 (11) :1767-1777
[16]   One-pot reaction to synthesize biocompatible magnetite nanoparticles [J].
Li, Z ;
Wei, L ;
Gao, MY ;
Lei, H .
ADVANCED MATERIALS, 2005, 17 (08) :1001-+
[17]   Magnetically targeted thrombolysis with recombinant tissue plasminogen activator bound to polyacrylic acid-coated nanoparticles [J].
Ma, Yunn-Hwa ;
Wu, Siao-Yun ;
Wu, Tony ;
Chang, Yeu-Jhy ;
Hua, Mu-Yi ;
Chen, Jyh-Ping .
BIOMATERIALS, 2009, 30 (19) :3343-3351
[18]   Superparamagnetic iron oxide nanoparticles (SPIONs): Development, surface modification and applications in chemotherapy [J].
Mahmoudi, Morteza ;
Sant, Shilpa ;
Wang, Ben ;
Laurent, Sophie ;
Sen, Tapas .
ADVANCED DRUG DELIVERY REVIEWS, 2011, 63 (1-2) :24-46
[19]   PREPARATION OF AQUEOUS MAGNETIC LIQUIDS IN ALKALINE AND ACIDIC MEDIA [J].
MASSART, R .
IEEE TRANSACTIONS ON MAGNETICS, 1981, 17 (02) :1247-1248
[20]   Transduction of full-length TAT fusion proteins into mammalian cells:: TAT-p27Kip1 induces cell migration [J].
Nagahara, H ;
Vocero-Akbani, AM ;
Snyder, EL ;
Ho, A ;
Latham, DG ;
Lissy, NA ;
Becker-Hapak, M ;
Ezhevsky, SA ;
Dowdy, SF .
NATURE MEDICINE, 1998, 4 (12) :1449-1452
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