High resolution melting PCR to differentiate Mycobacterium avium subsp paratuberculosis"cattle type" and "sheep type"

被引：10
作者：
Douarre, Pierre E.
Cashman, William [2 ]
Buckley, Jim [2 ]
Coffey, Aidan
O'Mahony, Jim M. [1 ]
机构：
[1] Cork Inst Technol, Dept Biol, Cork, Ireland
[2] Cork Cty Council, Vet Dept, Cork, Ireland
来源：
JOURNAL OF MICROBIOLOGICAL METHODS | 2012年 / 88卷 / 01期
关键词：
Mycobacterium avium subsp paratuberculosis; Genotyping; PCR; High resolution melting; MOLECULAR CHARACTERIZATION; GENETIC DIVERSITY; IDENTIFICATION; STRAINS;
D O I：
10.1016/j.mimet.2011.10.009
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
In this study, we describe a novel HRM-PCR assay that clearly differentiates the two main types of Mycobacterium avium subsp. paratuberculosis (cattle and sheep) based on the polymorphic variation of a previously described tandem repeat. This modern genotyping technique has several advantages over alternative methods, including cost, ease of use and rapidity. (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码：172 / 174
页数：3
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