Protocol for live-cell imaging during Tumor Treating Fields treatment with Inovitro Live

被引：4

作者：

Slangen, Paul L. G. ^{[1
]}

Porat, Yaara ^{[2
]}

Mertz, Marjolijn ^{[3
]}

Broek, Bram ^{[4
]}

Jalink, Kees ^{[4
]}

de Gooijer, Mark C. ^{[5
]}

van Tellingen, Olaf ^{[5
]}

Borst, Gerben R. ^{[1
,6
,7
]}

机构：

[1] Netherlands Canc Inst, Dept Radiat Oncol, Plesmanlaan 121, NL-1066 CX Amsterdam, Netherlands

[2] Novocure, Haifa, Israel

[3] Netherlands Canc Inst, Bioimaging Facil, Plesmanlaan 121, NL-1066 CX Amsterdam, Netherlands

[4] Netherlands Canc Inst, Div Cell Biol, Plesmanlaan 121, NL-1066 CX Amsterdam, Netherlands

[5] Netherlands Canc Inst, Div Pharmacol, Plesmanlaan 121, NL-1066 CX Amsterdam, Netherlands

[6] Christie NHS Fdn Trust, Wilmslow Rd, Manchester M20 4BX, England

[7] Univ Manchester, Fac Biol Med & Hlth, Sch Med Sci, Div Canc Sci, 555 Wilmslow Rd, Manchester M20 4GJ, England

来源：

STAR PROTOCOLS | 2022年 / 3卷 / 02期

关键词：

Cancer; Cell Biology; Cell culture; Microscopy;

D O I：

10.1016/j.xpro.2022.101246

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Tumor Treating Fields (TTFields) are an FDA-approved anticancer treatment using alternating electric fields. Here, we present a protocol to perform live-cell imaging (LCI) of cells during TTFields treatment with the Inovitro LiveTM system. The setup we describe dissipates TTFields-related heat production and can be used in conjunction with any LCI-compatible microscope setup. This approach will enable further elucidation of TTFields' mechanism of action at the molecular level and facilitate the development of promising combination strategies.

引用

页数：11

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