Single-molecule microscopy of the green fluorescent protein using simultaneous two-color excitation

被引：0
作者：
Jung, G
Wiehler, J
Steipe, B
Brauchle, C
Zumbusch, A
机构：
[1] Univ Munich, Lehrstuhl Phys Chem 1, D-81377 Munich, Germany
[2] Univ Munich, Genzentrum, D-81377 Munich, Germany
来源：
CHEMPHYSCHEM | 2001年 / 2卷 / 06期
关键词：
fluorescence; green fluorescent protein; photochemistry; proteins; single-molecule microscopy;
D O I：
10.1002/1439-7641(20010618)2:6<392::AID-CPHC392>3.0.CO;2-7
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
[No abstract available]
引用
收藏
页码：392 / 396
页数：5
相关论文
共 24 条
[1]   DETECTION AND SPECTROSCOPY OF SINGLE PENTACENE MOLECULES IN A PARA-TERPHENYL CRYSTAL BY MEANS OF FLUORESCENCE EXCITATION [J].
AMBROSE, WP ;
BASCHE, T ;
MOERNER, WE .
JOURNAL OF CHEMICAL PHYSICS, 1991, 95 (10) :7150-7163
[2]   Single-molecule fluorescence resonant energy transfer in calcium concentration dependent cameleon [J].
Brasselet, S ;
Peterman, EJG ;
Miyawaki, A ;
Moerner, WE .
JOURNAL OF PHYSICAL CHEMISTRY B, 2000, 104 (15) :3676-3682
[3]   Structural basis for dual excitation and photoisomerization of the Aequorea victoria green fluorescent protein [J].
Brejc, K ;
Sixma, TK ;
Kitts, PA ;
Kain, SR ;
Tsien, RY ;
Ormo, M ;
Remington, SJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (06) :2306-2311
[4]   Probing single molecules in single living cells [J].
Byassee, TA ;
Chan, WCW ;
Nie, SM .
ANALYTICAL CHEMISTRY, 2000, 72 (22) :5606-5611
[5]   Ultra-fast excited state dynamics in green fluorescent protein: Multiple states and proton transfer [J].
Chattoraj, M ;
King, BA ;
Bublitz, GU ;
Boxer, SG .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (16) :8362-8367
[6]   UNDERSTANDING, IMPROVING AND USING GREEN FLUORESCENT PROTEINS [J].
CUBITT, AB ;
HEIM, R ;
ADAMS, SR ;
BOYD, AE ;
GROSS, LA ;
TSIEN, RY .
TRENDS IN BIOCHEMICAL SCIENCES, 1995, 20 (11) :448-455
[7]   On/off blinking and switching behaviour of single molecules of green fluorescent protein [J].
Dickson, RM ;
Cubitt, AB ;
Tsien, RY ;
Moerner, WE .
NATURE, 1997, 388 (6640) :355-358
[8]   Real-time light-driven dynamics of the fluorescence emission in single green fluorescent protein molecules [J].
Garcia-Parajo, MF ;
Segers-Nolten, GMJ ;
Veerman, JA ;
Greve, J ;
van Hulst, NF .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (13) :7237-7242
[9]   Tracking single proteins within cells [J].
Goulian, M ;
Simon, SM .
BIOPHYSICAL JOURNAL, 2000, 79 (04) :2188-2198
[10]   Dynamics of fluorescence fluctuations in green fluorescent protein observed by fluorescence correlation spectroscopy [J].
Haupts, U ;
Maiti, S ;
Schwille, P ;
Webb, WW .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (23) :13573-13578
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