Hepatitis C Virus RNA Functionally Sequesters miR-122

被引：277

作者：

Luna, Joseph M. ^{[1
,2
,3
]}

Scheel, Troels K. H. ^{[1
,4
,5
,6
]}

Danino, Tal ^{[1
,7
]}

Shaw, Katharina S. ^{[1
]}

Mele, Aldo ^{[2
,3
]}

Fak, John J. ^{[2
,3
]}

Nishiuchi, Eiko ^{[1
]}

Takacs, Constantin N. ^{[1
,8
]}

Catanese, Maria Teresa ^{[1
]}

de Jong, Ype P. ^{[1
,9
]}

Jacobson, Ira M. ^{[9
]}

Rice, Charles M. ^{[1
]}

Darnell, Robert B. ^{[2
,3
,10
]}

机构：

[1] Rockefeller Univ, Ctr Study Hepatitis C, Lab Virol & Infect Dis, New York, NY 10065 USA

[2] Rockefeller Univ, Lab Mol Neurooncol, New York, NY 10065 USA

[3] Rockefeller Univ, Howard Hughes Med Inst, New York, NY 10065 USA

[4] Copenhagen Univ Hosp, Dept Infect Dis, Copenhagen Hepatitis C Program CO HEP, Hvidovre, Denmark

[5] Copenhagen Univ Hosp, Clin Res Ctr, Hvidovre, Denmark

[6] Univ Copenhagen, Fac Hlth & Med Sci, Dept Int Hlth Immunol & Microbiol, DK-2200 Copenhagen N, Denmark

[7] MIT, Hlth Sci & Technol, Cambridge, MA 02139 USA

[8] Rockefeller Univ, Lab Cellular Biophys, New York, NY 10065 USA

[9] Weill Cornell Med Coll, Div Gastroenterol & Hepatol, Ctr Study Hepatitis C, New York, NY 10065 USA

[10] New York Genome Ctr, New York, NY 10013 USA

来源：

CELL | 2015年 / 160卷 / 06期

关键词：

SINGLE-NUCLEOTIDE RESOLUTION; HITS-CLIP; CERNA HYPOTHESIS; BINDING PROTEIN; MICRORNA; IDENTIFICATION; ARGONAUTE; INFECTION; GENES; SITES;

D O I：

10.1016/j.cell.2015.02.025

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Hepatitis C virus (HCV) uniquely requires the liver-specific microRNA-122 for replication, yet global effects on endogenous miRNA targets during infection are unexplored. Here, high-throughput sequencing and crosslinking immunoprecipitation (HITS-CLIP) experiments of human Argonaute (AGO) during HCV infection showed robust AGO binding on the HCV 5'UTR at known and predicted miR-122 sites. On the human transcriptome, we observed reduced AGO binding and functional mRNA de-repression of miR-122 targets during virus infection. This miR-122 "sponge'' effect was relieved and redirected to miR-15 targets by swapping the miRNA tropism of the virus. Single-cell expression data from reporters containing miR-122 sites showed significant derepression during HCV infection depending on expression level and site number. We describe a quantitative mathematical model of HCV-induced miR-122 sequestration and propose that such miR-122 inhibition by HCV RNA may result in global derepression of host miR-122 targets, providing an environment fertile for the long-term oncogenic potential of HCV.

引用

页码：1099 / 1110

页数：12

共 44 条

[21] Selective Degradation of Host MicroRNAs by an Intergenic HCMV Noncoding RNA Accelerates Virus Production [J].

Lee, Sanghyun ;

Song, Jaewon ;

Kim, Sungchul ;

Kim, Jongkyu ;

Hong, Yujin ;

Kim, Youngkyun ;

Kim, Donghyun ;

Baek, Daehyun ;

Ahn, Kwangseog .

CELL HOST & MICROBE, 2013, 13 (06) :678-690

[22] miR-122 regulates collagen production via targeting hepatic stellate cells and suppressing P4HA1 expression [J].

Li, Jiang ;

Ghazwani, Mohammed ;

Zhang, Yifei ;

Lu, Jianqin ;

Li, Jilong ;

Fan, Jie ;

Gandhi, Chandrashekhar R. ;

Li, Song .

JOURNAL OF HEPATOLOGY, 2013, 58 (03) :522-528

[23] MicroRNA-122 antagonism against hepatitis C virus genotypes 1-6 and reduced efficacy by host RNA insertion or mutations in the HCV 5′ UTR [J].

Li, Yi-Ping ;

Gottwein, Judith M. ;

Scheel, Troels K. ;

Jensen, Tanja B. ;

Bukh, Jens .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2011, 108 (12) :4991-4996

[24] Competing and noncompeting activities of miR-122 and the 5′ exonuclease Xrn1 in regulation of hepatitis C virus replication [J].

Li, You ;

Masaki, Takahiro ;

Yamane, Daisuke ;

McGivern, David R. ;

Lemon, Stanley M. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2013, 110 (05) :1881-1886

[25] Visualizing Hepatitis C Virus Infections in Human Liver by Two-Photon Microscopy [J].

Liang, Yuqiong ;

Shilagard, Tuya ;

Xiao, Shu-Yuan ;

Snyder, Ned ;

Lau, Daryl ;

Cicalese, Luca ;

Weiss, Heidi ;

Vargas, Gracie ;

Lemon, Stanley M. .

GASTROENTEROLOGY, 2009, 137 (04) :1448-1458

[26] Ptbp2 represses adult-specific splicing to regulate the generation of neuronal precursors in the embryonic brain [J].

Licatalosi, Donny D. ;

Yano, Masato ;

Fak, John J. ;

Mele, Aldo ;

Grabinski, Sarah E. ;

Zhang, Chaolin ;

Darnell, Robert B. .

GENES & DEVELOPMENT, 2012, 26 (14) :1626-1642

[27] Masking the 5′ terminal nucleotides of the hepatitis C virus genome by an unconventional microRNA-target RNA complex [J].

Machlin, Erica S. ;

Sarnow, Peter ;

Sagan, Selena M. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2011, 108 (08) :3193-3198

[28] Cell Culture-Produced Hepatitis C Virus Does Not Infect Peripheral Blood Mononuclear Cells [J].

Marukian, Svedana ;

Jones, Christopher T. ;

Andrus, Linda ;

Evans, Matthew J. ;

Ritola, Kimberly D. ;

Charles, Edgar D. ;

Rice, Charles M. ;

Dustin, Lynn B. .

HEPATOLOGY, 2008, 48 (06) :1843-1850

[29] Genes Involved in Viral Carcinogenesis and Tumor Initiation in Hepatitis C Virus-Induced Hepatocellular Carcinoma [J].

Mas, Valeria R. ;

Maluf, Daniel G. ;

Archer, Kellie J. ;

Yanek, Kenneth ;

Kong, Xiangrong ;

Kulik, Laura ;

Freise, Chris E. ;

Olthoff, Kim M. ;

Ghobria, Rafik M. ;

McIver, Paula ;

Fisher, Robert .

MOLECULAR MEDICINE, 2009, 15 (3-4) :85-94

[30] Mapping Argonaute and conventional RNA-binding protein interactions with RNA at single-nucleotide resolution using HITS-CLIP and CIMS analysis [J].

Moore, Michael J. ;

Zhang, Chaolin ;

Gantman, Emily Conn ;

Mele, Aldo ;

Darnell, Jennifer C. ;

Darnell, Robert B. .

NATURE PROTOCOLS, 2014, 9 (02) :263-293

← 1 2 3 4 5 →