Serological markers for Leishmania donovani infection in Nepal: agreement between direct agglutination test and rK39 ELISA

被引：17
|
作者：
Khanal, Basudha [2 ]
Rijal, Suman [2 ]
Ostyn, Bart
Picado, Albert [3 ]
Gidwani, Kamlesh [4 ]
Menten, Joris
Jacquet, Diane
Lejon, Veerle
Chappuis, Francois [5 ]
Boelaert, Marleen [1 ]
机构：
[1] Inst Trop Med, Dept Publ Hlth, B-2000 Antwerp, Belgium
[2] BP Koirala Inst Hlth Sci, Dharan, Nepal
[3] London Sch Hyg & Trop Med, London WC1, England
[4] Banaras Hindu Univ, Varanasi 221005, Uttar Pradesh, India
[5] Univ Hosp Geneva, Geneva, Switzerland
来源：
TROPICAL MEDICINE & INTERNATIONAL HEALTH | 2010年 / 15卷 / 11期
关键词：
visceral leishmaniasis; kala-azar; Leishmania donovani; infection markers; LINKED-IMMUNOSORBENT-ASSAY; INDIAN VISCERAL LEISHMANIASIS; RECOMBINANT K-39 ANTIGEN; KALA-AZAR; DIAGNOSIS; ANTIBODY; EPIDEMIOLOGY; DISEASE;
D O I：
10.1111/j.1365-3156.2010.02631.x
中图分类号：
R1 [预防医学、卫生学];
学科分类号：
1004 ; 120402 ;
摘要：
Visceral leishmaniasis (VL) is an important vector-borne disease caused by Leishmania donovani in the Indian subcontinent. The actual incidence and role of asymptomatic infections in the region are not well known. We used the direct agglutination test (DAT) and the rK39 ELISA as L. donovani infection markers in 10 VL endemic villages in Nepal. DAT titre distribution showed two subgroups in the population (infected and non-infected individuals), while rK39 did not. The agreement between both tests was moderate (kappa = 0.53; 95% CI 0.49-0.57). More research is needed to develop validated markers for Leishmania infection.
引用
收藏
页码：1390 / 1394
页数：5
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