Galectin-3-induced cell spreading and motility relies on distinct signaling mechanisms compared to fibronectin

被引:8
作者
More, Shyam K. [1 ]
Chiplunkar, Shubhada V. [1 ]
Kalraiya, Rajiv D. [1 ]
机构
[1] Tata Mem Hosp, ACTREC, Sect 22, Kharghar 410210, Navi Mumbai, India
关键词
Galectin-3; Fibronectin; Microfilaments; Lamellipodia; Cell spreading; Cell migration; PROTEIN-KINASE; GROWTH-FACTOR; N-GLYCANS; EXTRACELLULAR-MATRIX; MOLECULAR-BASIS; MELANOMA-CELLS; RHO GTPASES; MIGRATION; ADHESION; INTEGRIN;
D O I
10.1007/s11010-016-2706-1
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Secreted galectin-3 often gets incorporated into extracellular matrix and is utilized by cancer cells for spreading, movement, and metastatic dissemination. Here we investigate molecular mechanisms by which galectin-3 brings about these effects and compare it with fibronectin. Imaging of cells spread on fibronectin showed stress fibers throughout cell body, however, galectin-3-induced formation of parallel actin bundles in the lamellipodial region resulting in unique morphological features. FRAP analysis showed that the actin turnover in the lamellipodial region was much higher in cells spread on galectin-3 as compared to that on fibronectin. Rac1 activation is correlated with lamellipodial organization on both the substrates. Activation of Akt and Rac1, the regulators of actin dynamics, show inverse correlation with each other on both galectin-3 and fibronectin. Activation of Erk however, remained similar. Further, inhibition of activation of Akt and Erk inhibited spreading and motility of cells on galectin-3 but not on fibronectin. The results very comprehensively demonstrate distinct signaling pathways that regulate microfilament organization, lamellipodial structures, spreading, and movement of cells plated on galectin-3 as opposed to fibronectin.
引用
收藏
页码:179 / 191
页数:13
相关论文
共 58 条
[1]   Role of tumor cell surface lysosome-associated membrane protein-1 (LAMP1) and its associated carbohydrates in lung metastasis [J].
Agarwal, Akhil Kumar ;
Srinivasan, Nithya ;
Godbole, Rashmi ;
More, Shyam K. ;
Budnar, Srikanth ;
Gude, Rajiv P. ;
Kalraiya, Rajiv D. .
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY, 2015, 141 (09) :1563-1574
[2]   Metastatic cancer cell [J].
Bacac, Marina ;
Stamenkovic, Ivan .
ANNUAL REVIEW OF PATHOLOGY-MECHANISMS OF DISEASE, 2008, 3 :221-247
[3]  
BARONDES SH, 1994, J BIOL CHEM, V269, P20807
[4]   Galectin-3-and phospho-caveolin-1-dependent outside-in integrin signaling mediates the EGF motogenic response in mammary cancer cells [J].
Boscher, Cecile ;
Nabi, Ivan R. .
MOLECULAR BIOLOGY OF THE CELL, 2013, 24 (13) :2134-2145
[5]   Galectin-3 Protein Regulates Mobility of N-cadherin and GM1 Ganglioside at Cell-Cell Junctions of Mammary Carcinoma Cells [J].
Boscher, Cecile ;
Zheng, Yu Zi ;
Lakshminarayan, Ramya ;
Johannes, Ludger ;
Dennis, James W. ;
Foster, Leonard J. ;
Nabi, Ivan R. .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2012, 287 (39) :32940-32952
[6]   Molecular aspects of tumor cell migration and invasion [J].
Bozzuto, Giuseppina ;
Ruggieri, Paola ;
Molinari, Agnese .
ANNALI DELL ISTITUTO SUPERIORE DI SANITA, 2010, 46 (01) :66-80
[7]   Identification of p130Cas as a mediator of focal adhesion kinase-promoted cell migration [J].
Cary, LA ;
Han, DC ;
Polte, TR ;
Hanks, SK ;
Guan, JL .
JOURNAL OF CELL BIOLOGY, 1998, 140 (01) :211-221
[8]   Molecular Origins of Cancer Molecular Basis of Metastasis [J].
Chiang, Anne C. ;
Massague, Joan .
NEW ENGLAND JOURNAL OF MEDICINE, 2008, 359 (26) :2814-2823
[9]   Extracellular galectin-3 induces MMP9 expression by activating p38 MAPK pathway via lysosome-associated membrane protein-1 (LAMP1) [J].
Dange, Manohar C. ;
Agarwal, Akhil Kumar ;
Kalraiya, Rajiv D. .
MOLECULAR AND CELLULAR BIOCHEMISTRY, 2015, 404 (1-2) :79-86
[10]   Galectin-3 expressed on different lung compartments promotes organ specific metastasis by facilitating arrest, extravasation and organ colonization via high affinity ligands on melanoma cells [J].
Dange, Manohar C. ;
Srinivasan, Nithya ;
More, Shyam K. ;
Bane, Sanjay M. ;
Upadhya, Archana ;
Ingle, Arvind D. ;
Gude, Rajiv P. ;
Mukhopadhyaya, Rabindranath ;
Kalraiya, Rajiv D. .
CLINICAL & EXPERIMENTAL METASTASIS, 2014, 31 (06) :661-673