Structure, organization and expression of two clustered cuticle protein genes during the metamorphosis of an insect, Tenebrio molitor

被引:19
作者
Rondot, I [1 ]
Quennedey, B [1 ]
Delachambre, J [1 ]
机构
[1] Univ Bourgogne, UMR CNRS 5548, Dijon, France
来源
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1998年 / 254卷 / 02期
关键词
gene duplication; cuticle protein gene; insect; metamorphosis; gene expression;
D O I
10.1046/j.1432-1327.1998.2540304.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A 4-kb DNA segment of Tenebrio molitor (Insecta, Coleoptera) genomic DNA containing two larval-pupal cuticular genes has been cloned and sequenced. These genes, transcribed in opposite directions, are related in DNA sequence and the proteins encoded are very similar. Each of them contains a single intron located inside the sequence encoding the signal peptide, and a conserved sequence at -200 bp from the mRNA start position. These similarities in sequence suggest that these genes have evolved by duplication followed by diversification and that they are members of a family of genes with a common ancestry. They are the first example of clustered genes in Tenebrio molitor.
引用
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页码:304 / 312
页数:9
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