The Comparison of Sponges and PLGA Scaffolds Impregnated with DBP on Growth Behaviors of Human Intervertebral Disc Cells

被引：6

作者：

Lee, Seon Kyoung ^{[1
,2
]}

Hong, Hee Kyung ^{[1
,2
]}

Kim, Su Jin ^{[1
,2
]}

Kim, Yong Ki ^{[1
,2
]}

Song, Vi Seul ^{[1
,2
]}

Ha, Yoon ^{[3
]}

Lee, Dongwon ^{[1
,2
]}

Khang, Gilson ^{[1
,2
]}

机构：

[1] Chonbuk Natl Univ, Dept BIN Fus Technol, Jeonju 561756, South Korea

[2] Chonbuk Natl Univ, Dept Polymer Nano Sci & Technol, Jeonju 561756, South Korea

[3] Yonsei Univ, Coll Med, Dept Neurosurg, Spine & Spinal Cord Inst, Seoul 120750, South Korea

来源：

POLYMER-KOREA | 2010年 / 34卷 / 05期

关键词：

DBP; PLGA; scaffold; sponge; H-disc; STROMAL CELLS; BONE; DIFFERENTIATION; PROLIFERATION; PROTEIN; LUMBAR;

D O I：

10.7317/pk.2010.34.5.398

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

We fabricated sponge and poly (lactide-co-glycolide) (PLGA) scaffolds impregnated demineralized bone particle(DBP) (DBP/PLGA) and investigated proper condition to proliferation and phenotype maintenance of intervertebral disc (IVD) cells by comparison between DBP/PLGA scaffold and DBP sponge. DBP/PLGA scaffolds were prepared by solvent casting/salt leaching. Human IVD cells were seeded in scaffolds of two types. Cell viability and proliferation according to scaffolds were analyzed by WST assay and SEM. RT-PCR was assessed to measure mRNA expression of aggrecan and type II collagen of human IVD cells. In WST assay results. cell viability in scaffolds impregnated DBP/PLGA scaffold were higher than DBP sponge. We could observe that disc cell mRNA expressed better in DBP/PLGA scaffold than DBP sponge. We concluded that the using of DBP/PLGA in terms of scaffold fabrication for bio-disc with human IVD cells is helpful growth of disc cells maintenance of phenotypes.

引用

页码：398 / 404

页数：7

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