The microbiota continuum along the female reproductive tract and its relation to uterine-related diseases

被引：652

作者：

Chen, Chen ^{[1
,2
]}

Song, Xiaolei ^{[1
,3
]}

Wei, Weixia ^{[4
,5
]}

Zhong, Huanzi ^{[1
,2
,6
]}

Dai, Juanjuan ^{[4
,5
]}

Lan, Zhou ^{[1
]}

Li, Fei ^{[1
,2
,3
]}

Yu, Xinlei ^{[1
,2
]}

Feng, Qiang ^{[1
,7
]}

Wang, Zirong ^{[1
]}

Xie, Hailiang ^{[1
]}

Chen, Xiaomin ^{[1
]}

Zeng, Chunwei ^{[1
]}

Wen, Bo ^{[1
,2
]}

Zeng, Liping ^{[4
,5
]}

Du, Hui ^{[4
,5
]}

Tang, Huiru ^{[4
,5
]}

Xu, Changlu ^{[1
,8
]}

Xia, Yan ^{[1
,3
]}

Xia, Huihua ^{[1
,2
,9
]}

Yang, Huanming ^{[1
,10
]}

Wang, Jian ^{[1
,10
]}

Wang, Jun ^{[1
,11
]}

Madsen, Lise ^{[1
,6
,12
]}

Brix, Susanne ^{[13
]}

Kristiansen, Karsten ^{[1
,6
]}

Xu, Xun ^{[1
,2
]}

Li, Junhua ^{[1
,2
,9
,14
]}

Wu, Ruifang ^{[4
,5
]}

Jia, Huijue ^{[1
,2
,9
,11
]}

机构：

[1] BGI Shenzhen, Shenzhen 518083, Peoples R China

[2] BGI Shenzhen, China Natl Genebank, Shenzhen 518120, Peoples R China

[3] Univ Chinese Acad Sci, BGI Educ Ctr, Shenzhen 518083, Peoples R China

[4] Peking Univ, Shenzhen Hosp, Shenzhen 518036, Peoples R China

[5] Shenzhen Key Lab Technol Early Diag Major Gynecol, Shenzhen 518036, Peoples R China

[6] Univ Copenhagen, Dept Biol, Lab Genom & Mol Biomed, Univ Pk 13, DK-2100 Copenhagen, Denmark

[7] BGI Shenzhen, Shenzhen Engn Lab Detect & Intervent Human Intest, Shenzhen 518083, Peoples R China

[8] Qingdao Univ, Qingdao Univ BGI Joint Innovat Coll, Qingdao 266071, Peoples R China

[9] BGI Shenzhen, Shenzhen Key Lab Human Commensal Microorganisms &, Shenzhen 518083, Peoples R China

[10] James D Watson Inst Genome Sci, Hangzhou 310000, Zhejiang, Peoples R China

[11] Macau Univ Sci & Technol, Taipa 999078, Macao, Peoples R China

[12] Natl Inst Nutr & Seafood Res, NIFES, Postboks 2029, N-5817 Bergen, Norway

[13] Tech Univ Denmark, Dept Biotechnol & Biomed, Bldg 221, DK-2800 Lyngby, Denmark

[14] South China Univ Technol, Sch Biosci & Biotechnol, Guangzhou 510006, Guangdong, Peoples R China

来源：

NATURE COMMUNICATIONS | 2017年 / 8卷

关键词：

METAGENOME-WIDE ASSOCIATION; VAGINAL MICROBIOME; GUT MICROBIOME; WOMEN; FLUID; COMMUNITIES; INFECTION; DYNAMICS; BACTERIA; SEQUENCE;

D O I：

10.1038/s41467-017-00901-0

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Reports on bacteria detected in maternal fluids during pregnancy are typically associated with adverse consequences, and whether the female reproductive tract harbours distinct microbial communities beyond the vagina has been a matter of debate. Here we systematically sample the microbiota within the female reproductive tract in 110 women of reproductive age, and examine the nature of colonisation by 16S rRNA gene amplicon sequencing and cultivation. We find distinct microbial communities in cervical canal, uterus, fallopian tubes and peritoneal fluid, differing from that of the vagina. The results reflect a microbiota continuum along the female reproductive tract, indicative of a non-sterile environment. We also identify microbial taxa and potential functions that correlate with the menstrual cycle or are over-represented in subjects with adenomyosis or infertility due to endometriosis. The study provides insight into the nature of the vagino-uterine microbiome, and suggests that surveying the vaginal or cervical microbiota might be useful for detection of common diseases in the upper reproductive tract.

引用

页数：11

共 47 条

[1] The Placenta Harbors a Unique Microbiome [J].

Aagaard, Kjersti ;

Ma, Jun ;

Antony, Kathleen M. ;

Ganu, Radhika ;

Petrosino, Joseph ;

Versalovic, James .

SCIENCE TRANSLATIONAL MEDICINE, 2014, 6 (237)

[2] A Study of the Vaginal Microbiome in Healthy Canadian Women Utilizing cpn60-Based Molecular Profiling Reveals Distinct Gardnerella Subgroup Community State Types [J].

Albert, Arianne Y. K. ;

Chaban, Bonnie ;

Wagner, Emily C. ;

Schellenberg, John J. ;

Links, Matthew G. ;

van Schalkwyk, Julie ;

Reid, Gregor ;

Hemmingsen, Sean M. ;

Hill, Janet E. ;

Money, Deborah .

PLOS ONE, 2015, 10 (08)

[3]

Anderson MJ, 2001, AUSTRAL ECOL, V26, P32, DOI 10.1111/j.1442-9993.2001.01070.pp.x

[4] Exploitation of the Medfly Gut Microbiota for the Enhancement of Sterile Insect Technique: Use of Enterobacter sp in Larval Diet-Based Probiotic Applications [J].

Augustinos, Antonios A. ;

Kyritsis, Georgios A. ;

Papadopoulos, Nikos T. ;

Abd-Alla, Adly M. M. ;

Caceres, Carlos ;

Bourtzis, Kostas .

PLOS ONE, 2015, 10 (09)

[5]

Bäckhed F, 2015, CELL HOST MICROBE, V17, P690, DOI [10.1016/j.chom.2015.04.004, 10.1016/j.chom.2015.05.012]

[6] Globular structure of human ovulatory cervical mucus [J].

Brunelli, Roberto ;

Papi, Massimiliano ;

Arcovito, Giuseppe ;

Bompiani, Adriano ;

Castagnola, Massimo ;

Parasassi, Tiziana ;

Sampaolese, Beatrice ;

Vincenzoni, Federica ;

De Spirito, Marco .

FASEB JOURNAL, 2007, 21 (14) :3872-3876

[7] QIIME allows analysis of high-throughput community sequencing data [J].

Caporaso, J. Gregory ;

Kuczynski, Justin ;

Stombaugh, Jesse ;

Bittinger, Kyle ;

Bushman, Frederic D. ;

Costello, Elizabeth K. ;

Fierer, Noah ;

Pena, Antonio Gonzalez ;

Goodrich, Julia K. ;

Gordon, Jeffrey I. ;

Huttley, Gavin A. ;

Kelley, Scott T. ;

Knights, Dan ;

Koenig, Jeremy E. ;

Ley, Ruth E. ;

Lozupone, Catherine A. ;

McDonald, Daniel ;

Muegge, Brian D. ;

Pirrung, Meg ;

Reeder, Jens ;

Sevinsky, Joel R. ;

Tumbaugh, Peter J. ;

Walters, William A. ;

Widmann, Jeremy ;

Yatsunenko, Tanya ;

Zaneveld, Jesse ;

Knight, Rob .

NATURE METHODS, 2010, 7 (05) :335-336

[8] Dermabacter vaginalis sp nov., isolated from human vaginal fluid [J].

Chang, Dong-Ho ;

Rhee, Moon-Soo ;

Kim, Byoung-Chan .

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2016, 66 :1881-1886

[9] Chronic endometritis: correlation among hysteroscopic, histologic, and bacteriologic findings in a prospective trial with 2190 consecutive office hysteroscopies [J].

Cicinelli, Ettore ;

De Ziegler, Dominique ;

Nicoletti, Roberto ;

Colafiglio, Giuseppe ;

Saliani, Nicola ;

Resta, Leonardo ;

Rizzi, Donatella ;

De Vito, Danila .

FERTILITY AND STERILITY, 2008, 89 (03) :677-684

[10] Quantitative determination by real-time PCR of four vaginal Lactobacillus species, Gardnerella vaginalis and Atopobium vaginae indicates an inverse relationship between L. gasseri and L. iners [J].

De Backer, Ellen ;

Verhelst, Rita ;

Verstraelen, Hans ;

Alqumber, Mohammed A. ;

Burton, Jeremy P. ;

Tagg, John R. ;

Temmerman, Marleen ;

Vaneechoutte, Mario .

BMC MICROBIOLOGY, 2007, 7 (1)

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