A wearable lab-on-a-patch platform with stretchable nanostructured biosensor for non-invasive immunodetection of biomarker in sweat

被引：124
作者：
Lee, Han-Byeol [1 ]
Meeseepong, Montri [2 ]
Tran Quang Trung [1 ]
Kim, Bo-Yeong [2 ]
Lee, Nae-Eung [1 ,2 ,3 ,4 ,5 ]
机构：
[1] Sungkyunkwan Univ, Sch Adv Mat Sci & Engn, 2066 Seobu Ro, Suwon 16419, Gyunggi Do, South Korea
[2] Sungkyunkwan Univ, SAINT, 2066 Seobu Ro, Suwon 16419, Gyunggi Do, South Korea
[3] Sungkyunkwan Univ, SAIHST, 2066 Seobu Ro, Suwon 16419, Gyunggi Do, South Korea
[4] Sungkyunkwan Univ, IQB, 2066 Seobu Ro, Suwon 16419, Gyunggi Do, South Korea
[5] Sungkyunkwan Univ, BICS, 2066 Seobu Ro, Suwon 16419, Gyunggi Do, South Korea
来源：
BIOSENSORS & BIOELECTRONICS | 2020年 / 156卷
基金：
新加坡国家研究基金会;
关键词：
Stretchable biosensor; Microfluidics; Immunodetection; Wearable device; 3D nanostructured impedimetric biosensor; CHEMICAL SENSORS; RAPID DETECTION; MICROFLUIDICS; CHALLENGES; BANDAGE; SYSTEM; FUTURE; SKIN;
D O I：
10.1016/j.bios.2020.112133
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
Conformable, wearable biosensor-integrated systems are a promising approach to non-invasive and quantitative on-body detection of biomarkers in body fluids. However, realizing such a system has been slowed by the difficulty of fabricating a soft affinity-based biosensor patch capable of precise on-body fluid handling with minimal wearer intervention and a simple measurement protocol. Herein, we demonstrate a conformable, wearable labon-a-patch (LOP) platform composed of a stretchable, label-free, impedimetric biosensor and a stretchable microfluidic device for on-body detection of the hormone biomarker, cortisol. The all-in-one, stretchable microfluidic device can precisely collect and deliver sweat for cortisol quantitation and offers one-touch operation of reagent delivery for simultaneous electrochemical signal generation and washing. Three-dimensional nanostructuring of the Au working electrode enables the high sensitivity required to detect the pM-levels of cortisol in sweat. Our integrated LOP detected sweat cortisol quantitatively and accurately during exercise. This LOP will open a new horizon for non-invasive, highly sensitive, and quantitative on-body immunodetection for wearable personal diagnostics.
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页数：8
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