A wearable lab-on-a-patch platform with stretchable nanostructured biosensor for non-invasive immunodetection of biomarker in sweat

被引：124

作者：

Lee, Han-Byeol ^{[1
]}

Meeseepong, Montri ^{[2
]}

Tran Quang Trung ^{[1
]}

Kim, Bo-Yeong ^{[2
]}

Lee, Nae-Eung ^{[1
,2
,3
,4
,5
]}

机构：

[1] Sungkyunkwan Univ, Sch Adv Mat Sci & Engn, 2066 Seobu Ro, Suwon 16419, Gyunggi Do, South Korea

[2] Sungkyunkwan Univ, SAINT, 2066 Seobu Ro, Suwon 16419, Gyunggi Do, South Korea

[3] Sungkyunkwan Univ, SAIHST, 2066 Seobu Ro, Suwon 16419, Gyunggi Do, South Korea

[4] Sungkyunkwan Univ, IQB, 2066 Seobu Ro, Suwon 16419, Gyunggi Do, South Korea

[5] Sungkyunkwan Univ, BICS, 2066 Seobu Ro, Suwon 16419, Gyunggi Do, South Korea

来源：

BIOSENSORS & BIOELECTRONICS | 2020年 / 156卷

基金：

新加坡国家研究基金会;

关键词：

Stretchable biosensor; Microfluidics; Immunodetection; Wearable device; 3D nanostructured impedimetric biosensor; CHEMICAL SENSORS; RAPID DETECTION; MICROFLUIDICS; CHALLENGES; BANDAGE; SYSTEM; FUTURE; SKIN;

D O I：

10.1016/j.bios.2020.112133

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Conformable, wearable biosensor-integrated systems are a promising approach to non-invasive and quantitative on-body detection of biomarkers in body fluids. However, realizing such a system has been slowed by the difficulty of fabricating a soft affinity-based biosensor patch capable of precise on-body fluid handling with minimal wearer intervention and a simple measurement protocol. Herein, we demonstrate a conformable, wearable labon-a-patch (LOP) platform composed of a stretchable, label-free, impedimetric biosensor and a stretchable microfluidic device for on-body detection of the hormone biomarker, cortisol. The all-in-one, stretchable microfluidic device can precisely collect and deliver sweat for cortisol quantitation and offers one-touch operation of reagent delivery for simultaneous electrochemical signal generation and washing. Three-dimensional nanostructuring of the Au working electrode enables the high sensitivity required to detect the pM-levels of cortisol in sweat. Our integrated LOP detected sweat cortisol quantitatively and accurately during exercise. This LOP will open a new horizon for non-invasive, highly sensitive, and quantitative on-body immunodetection for wearable personal diagnostics.

引用

页数：8

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