Distinctive gene transduction efficiencies of commonly used viral vectors in the retina

被引：11

作者：

Zhang, Sheng-Hai ^{[1
]}

Wu, Ji-Hong ^{[1
]}

Wu, Xiao-Bing ^{[2
]}

Dong, Xiao-Yan ^{[2
]}

Liu, Xin-Jian ^{[1
]}

Li, Chuan-Yuan ^{[3
]}

Huang, Qian ^{[1
]}

机构：

[1] Shanghai Jiao Tong Univ, Peoples Hosp 1, Expt Res Ctr, Shanghai 200080, Peoples R China

[2] Natl Inst Viral Dis Control & Prevent, State Key Lab Mol Virol & Genet Engn, Beijing, Peoples R China

[3] Univ Colorado, Hlth Sci Ctr, Dept Radiat Oncol, Aurora, CO USA

来源：

CURRENT EYE RESEARCH | 2008年 / 33卷 / 01期

基金：

中国国家自然科学基金;

关键词：

gene transduction efficiency; retina; transgene expression pattern; viral vector; viral vector tropism;

D O I：

10.1080/02713680701799408

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

The transduction efficiency and cell tropism of viral vectors rAAV2/1, rAAV2, Ad5, Ad5/F35, and Lentivirus were evaluated in retina. All viral vectors achieved efficient transduction in living rat retina. However, each vector showed distinctive efficiency in vitro especially for rAAV2/1, which displayed poor transduction in cultured retinal cells. Distinctive cell-specific GFP expression was observed in vivo and in vitro for the same viral vector. The cell-specific tropism was not strictly correlated with the correspondent distribution of viral receptors in retina. These results provided important insights into the selection of appropriate vectors when specific retinal diseases are considered for gene therapy.

引用

页码：81 / 90

页数：10

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