Generation of a heterozygous SOX10 knockout human embryonic stem cell line using CRISPR/Cas9 technology

被引：0

作者：

Wang, Tao ^{[1
,2
]}

Zhang, Jinli ^{[3
]}

Liu, Jia ^{[4
]}

Lai, Xingqiang ^{[5
]}

机构：

[1] Sun Yat Sen Univ, Key Lab Stem Cells & Tissue Engn, Ctr Stem Cell Biol & Tissue Engn, Zhongshan Sch Med,Minist Educ, Guangzhou, Guangdong, Peoples R China

[2] Sun Yat Sen Univ, Zhongshan Med Sch, Dept Biochem, Guangzhou, Guangdong, Peoples R China

[3] Jinan Univ, Guangzhou Red Cross Hosp, Guangzhou Inst Traumat Surg, Med Coll, Guangzhou, Guangdong, Peoples R China

[4] Sun Yat Sen Univ, VIP Med Serv Ctr, Affiliated Hosp 3, Guangzhou, Peoples R China

[5] Sun Yat Sen Univ, Affiliated Hosp 8, Cardiovasc Dept, Shenzhen, Guangdong, Peoples R China

来源：

STEM CELL RESEARCH | 2021年 / 57卷

基金：

中国国家自然科学基金;

关键词：

D O I：

10.1016/j.scr.2021.102567

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

SOX10 is one of the master transcription factors in neural crest development. Human SOX10 mutations are associated with Waardenburg syndrome type 4 (Waardenburg-Shah, WS4), which can be inherited in both autosomal dominant and recessive patterns. Here, the human embryonic stem cell (hESC) line, H9, was used to generate a heterozygous SOX10 knockout cell line as the in vitro model of WS4 by CRISPR/Cas9-mediated gene targeting. This cell line may represent a valuable tool for uncovering the pathogenesis of WS4.

引用

页数：5

共 50 条

[41] Generation of SHMT2 knockout human embryonic stem cell line (WAe009-A-67) using CRISPR/Cas9 technique [J].

Li, Jisheng ;

Zhang, Bowen ;

Gan, Min ;

Li, Yunxing ;

He, Lijuan ;

Yue, Wen ;

Qiao, Haixuan ;

Pei, Xuetao ;

Li, Yanhua .

STEM CELL RESEARCH, 2021, 57

[42] Generation of a KCNJ11 homozygous knockout human embryonic stem cell line WAe001-A-12 using CRISPR/Cas9 [J].

Yuan, Fang ;

Guo, Dongsheng ;

Gaob, Ge ;

Liu, Yanli ;

Xu, Yingying ;

Wu, Yuhang ;

Yang, Fan ;

Ke, Xinrong ;

Lai, Keyu ;

Hong, Liangqing ;

Li, Yin-xiong .

STEM CELL RESEARCH, 2017, 24 :89-93

[43] Generation of a NONO homozygous knockout human induced pluripotent stem cell line by CRISPR/Cas9 editing [J].

Yi, Tong ;

Bai, Rui ;

Sun, Hairui ;

Lan, Feng ;

Zhang, Hongjia ;

He, Yihua .

STEM CELL RESEARCH, 2020, 47

[44] Generation of a TLE1 homozygous knockout human embryonic stem cell line using CRISPR-Cas9 [J].

Herring, Amanda ;

Messana, Angelica ;

Bara, Anne M. ;

Hazelbaker, Dane Z. ;

Eggan, Kevin ;

Barrett, Lindy E. .

STEM CELL RESEARCH, 2016, 17 (02) :430-432

[45] Generation of an NCS1 gene knockout human induced pluripotent stem cell line using CRISPR/Cas9 [J].

Maierhof, Smilla K. ;

Schinke, Christian ;

Cernoch, Janine ;

Hew, Lois ;

Bruske, Laura Pauline ;

Vallone, Valeria Fernandez ;

Fischer, Kristin ;

Stachelscheid, Harald ;

Huehnchen, Petra ;

Endres, Matthias ;

Diecke, Sebastian ;

Telugu, Narasimha Swamy ;

Boehmerle, Wolfgang .

STEM CELL RESEARCH, 2023, 73

[46] Generation of a TLR7 homozygous knockout human induced pluripotent stem cell line using CRISPR/Cas9 [J].

Han, Hyeong-jun ;

Seo, Hyang-Hee ;

Han, Hyo-Won ;

Kim, Jung-Hyun .

STEM CELL RESEARCH, 2019, 40

[47] Generation of an ELTD1 knockout human embryonic stem cell line by the iCRISPR/Cas9 system [J].

Luo, Qian ;

Wei, Cong ;

Long, Yan ;

Zhang, Meng ;

Shan, Wei ;

Li, Honghu ;

Cai, Shuyang ;

Xu, Yulin ;

Qian, Pengxu ;

Huang, He .

STEM CELL RESEARCH, 2021, 53

[48] Generation of an RNF1-deficient human pluripotent stem cell line using CRISPR/Cas9 technology [J].

Zhang, Di ;

Zhou, Min ;

Zhang, Yanqi ;

Shan, Yongli ;

Pan, Guangjin .

STEM CELL RESEARCH, 2022, 62

[49] Generation of a TLR2 homozygous knockout human embryonic stem cell line WAe001-A-64 using CRISPR/Cas9 editing [J].

Getachew, Anteneh ;

Yang, Zhen ;

Huang, Xinping ;

Wu, Feima ;

Liu, Yan Ying ;

Yan, Chen ;

Yang, Fan ;

Li, Yinxiong .

STEM CELL RESEARCH, 2021, 54

[50] Construction of a GLI3 compound heterozygous knockout human embryonic stem cell line WAe001-A-20 by CRISPR/Cas9 editing [J].

Wei, Ruzhi ;

Yuan, Fang ;

Wu, Yuhang ;

Liu, Yanli ;

You, Kai ;

Yang, Zhen ;

Chen, Yan ;

Getachew, Anteneh ;

Wang, Ning ;

Xu, Yingying ;

Zhuang, Yuanqi ;

Yang, Fan ;

Li, Yin-xiong .

STEM CELL RESEARCH, 2018, 32 :139-144

← 1 2 3 4 5 →