Generation of a heterozygous SOX10 knockout human embryonic stem cell line using CRISPR/Cas9 technology

被引：0
作者：
Wang, Tao [1 ,2 ]
Zhang, Jinli [3 ]
Liu, Jia [4 ]
Lai, Xingqiang [5 ]
机构：
[1] Sun Yat Sen Univ, Key Lab Stem Cells & Tissue Engn, Ctr Stem Cell Biol & Tissue Engn, Zhongshan Sch Med,Minist Educ, Guangzhou, Guangdong, Peoples R China
[2] Sun Yat Sen Univ, Zhongshan Med Sch, Dept Biochem, Guangzhou, Guangdong, Peoples R China
[3] Jinan Univ, Guangzhou Red Cross Hosp, Guangzhou Inst Traumat Surg, Med Coll, Guangzhou, Guangdong, Peoples R China
[4] Sun Yat Sen Univ, VIP Med Serv Ctr, Affiliated Hosp 3, Guangzhou, Peoples R China
[5] Sun Yat Sen Univ, Affiliated Hosp 8, Cardiovasc Dept, Shenzhen, Guangdong, Peoples R China
来源：
STEM CELL RESEARCH | 2021年 / 57卷
基金：
中国国家自然科学基金;
关键词：
D O I：
10.1016/j.scr.2021.102567
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
SOX10 is one of the master transcription factors in neural crest development. Human SOX10 mutations are associated with Waardenburg syndrome type 4 (Waardenburg-Shah, WS4), which can be inherited in both autosomal dominant and recessive patterns. Here, the human embryonic stem cell (hESC) line, H9, was used to generate a heterozygous SOX10 knockout cell line as the in vitro model of WS4 by CRISPR/Cas9-mediated gene targeting. This cell line may represent a valuable tool for uncovering the pathogenesis of WS4.
引用
收藏
页数：5
相关论文
共 50 条
[21]   Generation of a homozygous HDAC6 knockout human embryonic stem cell line by CRISPR/Cas9 editing [J].
Xie, Liangqun ;
Huang, Jingrui ;
Li, Xun ;
Dai, Lei ;
Lin, Xinxiu ;
Zhang, Jiejie ;
Luo, Jiefeng ;
Zhang, Weishe .
STEM CELL RESEARCH, 2019, 41
[22]   Generation of a Bag1 homozygous knockout mouse embryonic stem cell line using CRISPR/Cas9 [J].
Tang, Cheng-cheng ;
Shan, Li Pik ;
Wang, Wu-ming ;
Lu, Gang ;
Tare, Rahul S. ;
Lee, Kenneth Ka Ho .
STEM CELL RESEARCH, 2017, 21 :29-31
[23]   Generation of a heterozygous SCN5A knockout human induced pluripotent stem cell line by CRISPR/Cas9 edition [J].
Gizon, Marie ;
Duboscq-Bidot, Laetitia ;
El Kassar, Lina ;
Bobin, Pierre ;
Ader, Flavie ;
Giraud-Triboult, Karine ;
Charron, Philippe ;
Villard, Eric ;
Fontaine, Vincent ;
Neyroud, Nathalie .
STEM CELL RESEARCH, 2022, 60
[24]   Generation of a homozygous GRIN2A gene knockout human embryonic stem cell line using CRISPR/Cas9 system [J].
Luo, Xian ;
Yang, Kaiming ;
Jiang, Meiling ;
Chen, Tao ;
Chi, Yue ;
Ma, Bingxiu ;
Lai, Liangxue ;
Zou, Qingjian .
STEM CELL RESEARCH, 2023, 69
[25]   Generation of a USP9Y knockout human embryonic stem cell line with CRISPR-Cas9 technology [J].
Wei, Sai ;
Zhen, Yuting ;
Sun, Chao ;
Ma, Yanlin ;
Li, Qi ;
Wen, Luan .
STEM CELL RESEARCH, 2025, 82
[26]   An Efficient Method for Generation of Knockout Human Embryonic Stem Cells Using CRISPR/Cas9 System [J].
Bohaciakova, Dasa ;
Renzova, Tereza ;
Fedorova, Veronika ;
Barak, Martin ;
Kunova Bosakova, Michaela ;
Hampl, Ales ;
Cajanek, Lukas .
STEM CELLS AND DEVELOPMENT, 2017, 26 (21) :1521-1527
[27]   Generation of myoglobin (MB)-knockout human embryonic stem cell (hESC) line (KAIMRCe002-A-1S) using CRISPR/Cas9 technology [J].
Alowaysi, Maryam ;
Al-Shehri, Mohammad ;
Baadhaim, Moayad ;
AlZahrani, Hajar ;
Aboalola, Doaa ;
Daghestani, Mustafa ;
Hashem, Heba ;
Aljahdali, Rawan ;
Salem, Rayan ;
Alharbi, Adel ;
Muharraq, Mohammed ;
Alghamdi, Khaled ;
Alsobiy, Fawaz ;
Zia, Asima ;
Lehmann, Robert ;
Tegner, Jesper ;
Alsayegh, Khaled .
STEM CELL RESEARCH, 2023, 71
[28]   Generation of a TPM1 homozygous knockout embryonic stem cell line by CRISPR/Cas9 editing [J].
Ma, Shuhong ;
Xu, Qi ;
Bai, Rui ;
Dong, Tao ;
Peng, Zhiping ;
Liu, Xujie .
STEM CELL RESEARCH, 2021, 55
[29]   Generation of two ERF gene knockout human embryonic stem cell lines using CRISPR/Cas9 system [J].
Si, Lihui ;
Yang, Ruiqi ;
Liu, Jian ;
Dong, Yubo ;
Zhang, Hongtao ;
Xu, Xiaohong .
STEM CELL RESEARCH, 2019, 41
[30]   Generation of a TLR7 heterozygous knockout line (WAe009-A-18) from human embryonic stem cells using CRISPR/Cas9 [J].
Seo, Hyang-Hee ;
Han, Hyo-Won ;
Kim, Jung-Hyun .
STEM CELL RESEARCH, 2020, 45
12345