Generation of a heterozygous SOX10 knockout human embryonic stem cell line using CRISPR/Cas9 technology

被引：0

作者：

Wang, Tao ^{[1
,2
]}

Zhang, Jinli ^{[3
]}

Liu, Jia ^{[4
]}

Lai, Xingqiang ^{[5
]}

机构：

[1] Sun Yat Sen Univ, Key Lab Stem Cells & Tissue Engn, Ctr Stem Cell Biol & Tissue Engn, Zhongshan Sch Med,Minist Educ, Guangzhou, Guangdong, Peoples R China

[2] Sun Yat Sen Univ, Zhongshan Med Sch, Dept Biochem, Guangzhou, Guangdong, Peoples R China

[3] Jinan Univ, Guangzhou Red Cross Hosp, Guangzhou Inst Traumat Surg, Med Coll, Guangzhou, Guangdong, Peoples R China

[4] Sun Yat Sen Univ, VIP Med Serv Ctr, Affiliated Hosp 3, Guangzhou, Peoples R China

[5] Sun Yat Sen Univ, Affiliated Hosp 8, Cardiovasc Dept, Shenzhen, Guangdong, Peoples R China

来源：

STEM CELL RESEARCH | 2021年 / 57卷

基金：

中国国家自然科学基金;

关键词：

D O I：

10.1016/j.scr.2021.102567

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

SOX10 is one of the master transcription factors in neural crest development. Human SOX10 mutations are associated with Waardenburg syndrome type 4 (Waardenburg-Shah, WS4), which can be inherited in both autosomal dominant and recessive patterns. Here, the human embryonic stem cell (hESC) line, H9, was used to generate a heterozygous SOX10 knockout cell line as the in vitro model of WS4 by CRISPR/Cas9-mediated gene targeting. This cell line may represent a valuable tool for uncovering the pathogenesis of WS4.

引用

页数：5

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