Dynamics of the PI3K-like protein kinase members ATM and DNA-PKcs at DNA double strand breaks

被引：28

作者：

Davis, Anthony J. ^{[1
]}

So, Sairei ^{[1
]}

Chen, David J. ^{[1
]}

机构：

[1] Univ Texas SW Med Ctr Dallas, Dept Radiat Oncol, Div Mol Radiat Biol, Dallas, TX 75390 USA

来源：

CELL CYCLE | 2010年 / 9卷 / 13期

基金：

美国国家卫生研究院;

关键词：

ATM; DNA-PKcs; DNA double strand breaks; autophosphorylation; live cell imaging; CATALYTIC SUBUNIT; AUTOPHOSPHORYLATION SITES; ATAXIA-TELANGIECTASIA; CHROMATIN-STRUCTURE; FACILITATES REPAIR; IN-VIVO; ACTIVATION; PHOSPHORYLATION; NBS1; CELL;

D O I：

10.4161/cc.9.13.12148

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The protein kinases ATM and DNA-PKcs play critical roles in the cellular response to DNA double strand breaks (DSBs). ATM and DNA-PKcs are activated in response to DSBs and play several important roles in propagation of the damage signal and for the repair of DNA damage. Recent work from several groups, including ours, has focused on studying the dynamics of each of these proteins at DSBs and the requirements and factors which play a role(s) in this process. The use of live cell imaging of fluorescently-tagged ATM and DNA-PKcs has allowed us to study the real-time response of these proteins to laser-generated DNA damage in vivo. Here, we will extensively discuss the behavior of the ATM and DNA-PKcs proteins at DSB sites.

引用

页码：2529 / 2536

页数：8

共 55 条

[1] Structural and functional analysis of Mre11-3 [J].

Arthur, LM ;

Gustausson, K ;

Hopfner, KP ;

Carson, CT ;

Stracker, TH ;

Karcher, A ;

Felton, D ;

Weitzman, MD ;

Tainer, J ;

Carney, JP .

NUCLEIC ACIDS RESEARCH, 2004, 32 (06) :1886-1893

[2] DNA damage activates ATM through intermolecular autophosphorylation and dimer dissociation [J].

Bakkenist, CJ ;

Kastan, MB .

NATURE, 2003, 421 (6922) :499-506

[3] Spatial organization of the mammalian genome surveillance machinery in response to DNA strand breaks [J].

Bekker-Jensen, S ;

Lukas, C ;

Kitagawa, R ;

Melander, F ;

Kastan, MB ;

Bartek, J ;

Lukas, J .

JOURNAL OF CELL BIOLOGY, 2006, 173 (02) :195-206

[4] Roles of ATM and NBS1 in chromatin structure modulation and DNA double-strand break repair [J].

Berkovich, Elijahu ;

Monnat, Raymond J., Jr. ;

Kastan, Michael B. .

NATURE CELL BIOLOGY, 2007, 9 (06) :683-U137

[5] Autophosphorylation-dependent remodeling of the DNA-dependent protein kinase catalytic subunit regulates ligation of DNA ends [J].

Block, WD ;

Yu, YP ;

Merkle, D ;

Gifford, JL ;

Ding, Q ;

Meek, K ;

Lees-Miller, SP .

NUCLEIC ACIDS RESEARCH, 2004, 32 (14) :4351-4357

[6] Role of non-homologous end joining (NHEJ) in maintaining genomic integrity [J].

Burma, Sandeep ;

Chen, Benjamin P. C. ;

Chen, David J. .

DNA REPAIR, 2006, 5 (9-10) :1042-1048

[7] DNA looping by Ku and the DNA-dependent protein kinase [J].

Cary, RB ;

Peterson, SR ;

Wang, JT ;

Bear, DG ;

Bradbury, EM ;

Chen, DJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (09) :4267-4272

[8] Independent roles for nibrin and Mre11-Rad50 in the activation and function of Atm [J].

Cerosaletti, K ;

Concannon, P .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (37) :38813-38819

[9] Autophosphorylation of the DNA-dependent protein kinase catalytic subunit is required for rejoining of DNA double-strand breaks [J].

Chan, DW ;

Chen, BPC ;

Prithivirajsingh, S ;

Kurimasa, A ;

Story, MD ;

Qin, J ;

Chen, DJ .

GENES & DEVELOPMENT, 2002, 16 (18) :2333-2338

[10] Cell cycle dependence of DNA-dependent protein kinase phosphorylation in response to DNA double strand breaks [J].

Chen, BPC ;

Chan, DW ;

Kobayashi, J ;

Burma, S ;

Asaithamby, A ;

Morotomi-Yano, K ;

Botvinick, E ;

Qin, J ;

Chen, DJ .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (15) :14709-14715

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