A New Protein of α-Amylase Activity from Lactococcus lactis

被引：11
|
作者：
Wasko, Adam [1 ]
Polak-Berecka, Magdalena [1 ]
Targonski, Zdzislaw [1 ]
机构：
[1] Univ Life Sci Lublin, Dept Biotechnol Human Nutr & Food Commod, PL-20704 Lublin, Poland
来源：
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | 2010年 / 20卷 / 09期
关键词：
alpha-Amylase; ALAB; Lactococcus lactis; gene transfer; evolutionary origin; LACTOBACILLUS-AMYLOVORUS; SP; NOV; PURIFICATION; STARCH; STRAINS; PLANTARUM; FERMENTUM; SEQUENCE; GENES;
D O I：
10.4014/jmb.1002.02005
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
An extracellular alpha-amylase from Lactococcus lactis IBB500 was purified and characterized. The optimum conditions for the enzyme activity were a pH of 4.5, temperature of 35 degrees C, and enzyme molecular mass of 121 kDa. The genome analysis and a plasmid curing experiment indicated that amy(+) genes were located in a plasmid of 30 kb. An analysis of the phylogenetic relationships strongly supported a hypothesis of horizontal gene transfer. A strong homology was found for the peptides with the sequence of alpha-amylases from Ralstonia pikettii and Ralstonia solanacearum. The protein with alpha-amylase activity purified in this study is the first one described for the Lactococcus lactis species, and this paper is the first report on a Lactococcus lactis strain belonging to the amylolytic lactic acid bacteria (ALAB).
引用
收藏
页码：1307 / 1313
页数：7
相关论文
共 50 条
  • [41] Biological response modifier activity of Lactococcus lactis 332
    Maeda, M
    Ueda, H
    Yamazaki, M
    Ohtsuka, M
    Doi, U
    YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN, 1998, 118 (04): : 150 - 157
  • [42] Immunostimulatory Activity of Lactococcus lactis subsp. lactis CAB701 Isolated from Jeju Cabbage
    Jeong, Huijin
    Kim, Suin
    Hwang, Un-Sik
    Choi, Hyukjoon
    Park, Young-Seo
    MICROORGANISMS, 2023, 11 (07)
  • [43] Inflammation-related pro-apoptotic activity of exopolysaccharides isolated from Lactococcus lactis subsp lactis
    Wu, Z.
    Wang, G.
    Pan, D.
    Guo, Y.
    Zeng, X.
    Sun, Y.
    Cao, J.
    BENEFICIAL MICROBES, 2016, 7 (05) : 761 - 768
  • [44] PROTEIN CHARACTERIZATION OF LACTOCOCCUS-LACTIS SSP LACTIS AND L-LACTIS SSP CREMORIS BACTERIOPHAGES ISOLATED FROM CHEESE WHEYS
    SHIEH, YSC
    LEDFORD, RA
    TORTORELLO, ML
    DUNNY, GM
    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1993, 40 (01) : 107 - 112
  • [45] Characterization of bacteriocins from two Lactococcus lactis subsp lactis isolates
    Akçelik, O
    Tükel, Ç
    Özcengiz, G
    Akçelik, M
    MOLECULAR NUTRITION & FOOD RESEARCH, 2006, 50 (03) : 306 - 313
  • [46] Characterization of Lactococcus lactis subsp lactis isolated from surface waters
    Svec, P.
    Sedlacek, I.
    FOLIA MICROBIOLOGICA, 2008, 53 (01) : 53 - 56
  • [47] Concentration and recovery of the bacteriocin nisin from Lactococcus lactis subsp lactis
    VantHul, JS
    Gibbons, WR
    BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, 1996, 24 : 251 - 256
  • [48] Characterization of N-deoxyribosyltransferase from Lactococcus lactis subsp lactis
    Miyamoto, Yukiko
    Masaki, Takeharu
    Chohnan, Shigeru
    BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 2007, 1774 (10): : 1323 - 1330
  • [49] PURIFICATION AND CHARACTERIZATION OF PULLULANASE FROM Lactococcus lactis
    Wasko, Adam
    Polak-Berecka, Magdalena
    Targonski, Zdzislaw
    PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, 2011, 41 (03): : 252 - 261
  • [50] Production and crystallization of α-phosphoglucomutase from Lactococcus lactis
    Nogly, Przemyslaw
    Castro, Rute
    de Rosa, Matteo
    Neves, Ana Rute
    Santos, Helena
    Archer, Margarida
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2012, 68 : 1113 - 1115
12345