Polymerase chain reaction identification of coagulase-negative staphylococci and of strain diversity and spread of Staphylococcus epidermidis in a major medical center in Lebanon

被引：3

作者：

Abdallah, Inaya M.

Araj, George F.

Matar, Ghassan M.

Abdelnour, George

Uwaydah, Marwan

Abdelnoor, Alexander M.

机构：

[1] Amer Univ Beirut, Dept Microbiol & Immunol, Beirut 11072020, Lebanon

[2] Amer Univ Beirut, Dept Pathol & Lab Med, Beirut 11072020, Lebanon

[3] Hop Notre Dame Des Socours, Byblos, Lebanon

来源：

INFECTION CONTROL AND HOSPITAL EPIDEMIOLOGY | 2006年 / 27卷 / 07期

关键词：

D O I：

10.1086/505093

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

A 2-step polymerase chain reaction ( PCR) assay and random amplification of polymorphic DNA ( RAPD) analysis, respectively, were assessed to identify coagulase-negative staphylococci organisms to the species level and to determine the strain diversity and spread of Staphylococcus epidermidis, the most frequently isolated species, in a medical center in Beirut, Lebanon. Our data indicated that PCR was faster and was more efficient in identifying S. epidermidis isolates than is conventional biochemical testing. RAPD analysis have shown that S. epidermidis strains were scattered across the different clinical services, demonstrating various clusters of infection in the medical center.

引用

页码：781 / 783

页数：3